<p style="text-align: justify;">The objectives of this researchwasto determine the effect of growing media composition on growth and production of two varieties of tomatoes in a hydroponic system. The research used Randomized Complate Block Design (RCBD) with two factors, they were tomato varieties and growing media composition. The tomato varieties were (Arthaloka, Permata) and growing media compotition were 100% husk (v/v), 75% husk + 25% bamboo leaves compost (v/v), 50% husks + 50% bamboo leaves compost (v/v), 25% husk + 75% bamboo leaves compost (v/v), and 100% bamboo leaves compost (v/v). Permata variety better than Arthaloka variety, based the higher number of flower and flower bunches, number of fruit, weight of fruit and grade of fruit. Bamboo leaves compost increased heigh of plant at 2-5 week after transplanting (WAT), number of leaves at 2-4 WAT, number of flower at 4-5 WAT, and 7-8 WAT, and number of flowers bunches at 5–11 WAT. The used of bamboo leaf compost 50% (v/v) and 75% (v/v) increased fruit number of tomato Permata variety at 7 WAT.</p><p>Keywords: rice husk, bamboo leaf compost, hydroponics, tomato production</p>
Abstract. Increasing of aquaculture production is playing an important contribution to increase of environmental and pathological problems in several aquaculture production centers. Therefore, prevention and control of diseases are now become a top priority for the sustainability of this industry. The objective of the present study was to evaluate the distribution of fish pathogen in some mariculture production centers in Riau Islands Province. The study was conducted from Febrari 2011 to December 2013. The study showed that Nodavirus and Iridovirus as a viral disease-causing agents were commonly found in marine fish farm. While Vibrio spp., Aeromonas spp. and Edwardsiella spp. were general pathogenic microorganism in marine and freshwater fish farms. Furthermore, there were also found various parasites such as Diplectanum sp., Gyrodactilus sp., Caligus sp., Trichodina sp., Rhexanella sp., Hirudinae sp., Benedenia sp. and Cylodonela sp. in various marine and freshwater fish production centers in the Riau Islands Province. Keywords: Monitoring; Fish diseases;Parasite; Bacteria; Virus. Abstrak. Peningkatan laju produksi perikanan budidaya secara umum berperan penting dalam peningkatan masalah lingkungan dan patogen di beberapa unit produksi budidaya. Oleh karena itu, pencegahan dan pengendalian penyakit saat ini menjadi prioritas untuk menjamin keberlanjutan industri ini. Penelitian ini bertujuan untuk melakukan monitoring dan menilai distribusi patogen pada beberapa sentra produksi perikanan laut di Provinsi Kepulauan Riau. Monitoing dilakukan mulai Februari 2011 sampai Desember 2013. Hasil penelitian menunjukkan bahwa Nodavirus dan Iridovirus merupakan agen penyebab penyakit virus yang umum ditemukan di budidaya ikan laut. Sementara Vibrio spp., Aeromonas spp. dan Edwardsiella spp. merupakan mikroorganisme patogen yang umum ditemukan di sentra budidaya ikan laut dan ikan air tawar. Hasil kajian monitoring juga menunjukkan bahwa Diplectanum sp., Gyrodactilus sp., Caligus sp., Trichodina sp., Rhexanella sp., Hirudinae sp., Benedenia sp. dan Cylodonela sp. merupakan parasit yang memiliki distribusi tinggi di berbagai sentra produksi ikan air laut dan tawar di Kepulauan Riau.Kata Kunci : Monitoring; penyakit ikan; ; parasit; bakteri; virus; Kepulauan Riau.
The orange clownfish, Amphiprion percula, is a popular fish in the global marine ornamental trade. In March 2021, several orange clown fish at Batam Mariculture Development Center exhibited lethargic behavior with wart-like nodules on the fins suggesting a viral disease infection. This study aimed to detect the suspected viral disease agent from the clownfish and determine its genotype based on the major capsid protein gene. The fish tissues with wart-like nodules were collected and fixed in 96% ethanol. The DNA was extracted from the tissues and used as the template for the amplification of the major capsid protein (MCP) and myristylated membrane protein (MMP) genes using polymerase chain reaction (PCR). The nucleotide sequences of the PCR products were analyzed for their homology using the Basic Local Alignment Search Tool (BLAST). Multiple alignments of the amino acid sequence of MCP were performed using MEGA-X to determine the genotype. The PCR amplification produced the expected bands for detecting MCP, MMP and DNAPol genes. These results indicated the presence of Lymphocystis disease virus (LCDV), designated as LCDV-Oc-Btm. The sequencing of MCP and MMP genes produced the 1221 and 407 nucleotides, respectively. The BLAST analysis showed the highest identity was obtained with the species of LCDV-1 (LCDV-Sa strain) at 91.04 % and 88.19 % for MCP and MMP, respectively. The UPGMA phylogenic tree showed LCDV-Oc-Btm differs from the existing genotype and can be assigned as a new genotype. This study concludes that LCDVOc-Btm is a novel species of lymphocystis disease virus.
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