DNA sequencing technology has a rich diverse history. Over the past several years, next-generation sequencing (NGS) has demonstrated its potential to accelerate research in the field of life sciences in general and forensic sciences in particular. Although the abilities of these new technologies allow cheap and comprehensive analyses within a short time, other important characteristics other than the technologies themselves (such as the quantity and quality of data) are generated. These characteristics present a set of challenges for experimental design, data analysis, and interpretation. Moreover, other improvements are needed to make NGS technology more attractive for routine forensics work. This review aims to shed light on the development and advancements of NGS from both historical and contemporary views. Advantages and limitations of NGS are mentioned and NGS in forensic sciences is summarized.
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