This study aimed to detect the infection rate of Toxoplasma gondii in 100 local breed domesticated chickens (Gullas gullas domesticus) by Real Time-PCR based on B1 gene and determined the effects of some factors (sex, age and areas) in this rate during the period 1/ 10 / 2019 till 31/3/ 2020. The total infection rate was 11% and a higher infection rate (17.24%) was found in females than males (2.38 %) with significant (P≤ 0.01) difference. A higher infection rate (16.32%) was recorded in young (<6 months) females while no infection rate (0.00%) was found in young males (<6 months). The adult females (>6 months) were showed a higher infection rate (22.22%) than adult males 4.76% (>6 months) with significant (P≤0.01) difference. A higher infection rate (23.33%) was recorded in AL-Baia area followed by Al-Malhani (6.66%), while no infection rate (0.00%) was found in Baghdad AL-Jadida with significant (P≤ 0.01) difference.
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