This study was carried out to study the beneficial role of organic acids and other beneficial compounds produced by Aspergillus japonicas and their effects on the egg production, egg quality, fertility, and hatchability as well as blood metabolites of quail breeders. A number of 150 mature 8-week of age Japanese quails (100 females and 50 males) were used and divided into 5 groups. The 1st, 2nd, 3rd, 4 th , and 5th groups were fed basal diet with 0 (control), 1, 2, 3, and 4 ml Aspergillus japonicas culture filtrate/kg diet, respectively. The use of Aspergillus japonicas filtrate improved egg number, egg mass and feed conversion ratio when compared to control. The use of Aspergillus japonicas filtrate quadratically improved feed conversion ratio when compared to control at all ages. Quail breeders fed 2 and 3 ml Aspergillus japonicas filtrate-treated diets consumed less feed than the other diets (0, 1, or 4 ml) during 8-12 week-old and the overall period. Supplementation of Aspergillus japonicas filtrate to quail diets improved fertility percentage during 12-16 and 8-16 week of age, the best level was 2 ml/kg of filtrate. Addition of Aspergillus japonicas filtrate to quail diets reduced the shell percentage and shell thickness, but increased the Haugh unit. The use of Aspergillus japonicas filtrate in quail breeder's diets tended to decline total cholesterol, triglyceride, LDL, and VLDL in plasma. Inclusion of Aspergillus japonicas filtrate in the diets of quail breeders improved the immune parameters. The activity of SOD and TAC was higher in Aspergillus japonicas filtrate groups than the control. In conclusion, supplemental Aspergillus japonicas filtrate could improve productive and reproductive performance, lipid profile, immunity, and antioxidant indices. HIGHLIGHTSUse of Aspergillus filtrate in quail diets improved egg production and egg mass. Use of Aspergillus japonicas filtrate to quail diets improved fertility percentage Use of Aspergillus filtrate in quail diets improved immunity and antioxidant indices Dietary addition of Aspergillus filtrate improved lipid profile.

This study was carried out to examine the beneficial role of organic acids and other beneficial compounds produced by Aspergillus japonicas and their effects on performance, carcase yield, digestive enzymes, intestinal microbiota and blood constituents of quail. 240 quails (7 days old) were allotted to 5 groups consisting of 48 quail chicks (4 replicates of 12 chicks). The first, second, third, fourth and fifth groups were fed basal diet with 0 (control), 1, 2, 3, and 4 mL A. japonicas culture filtrate/kg diet, respectively. The addition of A. japonicas culture filtrate up to 3 mL/kg feed increased body weight at 3 and 5 weeks-old and body weight gain from 1-3, 3-5 and 1-5 weeks, the best level was 3 mL/kg feed. Quails fed A. japonicas filtrate diets recorded the best feed conversion ratio in comparison with the control. Dietary addition of A. japonicas filtrate tended to decline triglyceride, LDL, and VLDL and increased HDL values in the plasma. Use of A. japonicas filtrate in diets up to 2 mL/kg improved the immune indices. The levels of SOD and TAC, GSH and CAT as well as digestive enzymes were improved due to A. japonicas filtrate supplementation compared to control. Plasma levels of MDA were decreased (p ¼ .0001) in the quails fed Aspergillus filtrate (1 or 2 mL/kg). Quails fed rations enriched with filtrate exhibited lower colonisation of Escherichia coli, coliform and Salmonella. In conclusion, supplemental A. japonicas culture filtrate could improve the performance, digestive enzymes, immunity, antioxidant indices and mitigate intestinal pathogens. HIGHLIGHTSUse of Aspergillus filtrate improved in quail diets performance and digestive enzymes. Use of Aspergillus filtrate in quail diets improved immunity and antioxidant indices. Dietary addition of Aspergillus filtrate mitigated intestinal pathogens.