Hypertriglyceridemia is condition when there is an increase of triglyceride levels in blood. Hypertriglyceridemia can be increased blood pressure and heart problems. One of the plants can be used as a medicine is kasturi citrus peel (Citrus japonica Thunb.). This study aimed to determine the effect of giving butano fraction and ethyl acetate fraction of kasturi citrus peel (Citrus japonica Thunb.) to triglyceride levels on male white rats (Rattus norvegicus). The parameters are triglyceride levels in serum of male white rat induced with high-fat feed containing quail egg yolk (10 ml/kgWB). This research used 25 male white rats that divided into 5 groups with 5 rats per group. Normal groups are not given any treatment, only standard feed. The negative group was induced by high-fat feed and suspension Na CMC, the positive group was given high-fat feed and atorvastatin suspension 0,9 mg/kgWB and treatment group was given high-fat feed induction and suspension of ethyl acetate and butanol fraction of kasturi citrus peel with doses 250 mg/kgBW, as much 1% of body weight wich given orally. The triglyceride levels are calculating by using colorimetri enzymatic method with a photometer Mindray® (BA-88A) at wavelength 510 nm. The data percentage change in triglyceride levels on day 29 to day 15 obtained were analyzed with one-way Analysis of Variance (ANOVA) and continued with the Post Hoc Tukey test for each fraction. The results showed that giving butanol fraction and ethyl acetate fraction of kasturi citrus peel (Citrus japonica Thunb.) at doses of 250 mg/kg body weight had an effect on the decrease in triglyceride levels in serum of white male rat indicated by a significant difference (p<0.05) with negative control and showed a decrease in triglyceride levels activity that was not significant difference (p>0.05) with atorvastatin and there is no significant difference between anti hypertriglyceride activity of butanol fraction and ethyl acetate fraction (p>0.05)
ABSTRAK: Telah dilakukan isolasi dan pengujian sitotoksik ekstrak etil asetat kulit batang meranti rambai (Shorea acuminata Dyer). Ekstraksi dilakukan dengan maserasi bertingkat dan uji sitotoksik dengan metode Brine Shrimp Lethality Test (BSLT). Isolasi dilakukan dengan kromatografi kolom memakai sistem gradient dan diperoleh senyawa D 1 , berupa amorf berwarna putih sebanyak 18 mg dengan titik leleh 280-282 o C. Berdasarkan hasil reaksi dengan reagen Liebermann-Buchard (yaitu warna orange kecoklatan) dan hasil karakterisasi dengan spektroskopi UV, IR, 1 H-RMI, dan 13 C-RMI, maka dapat disimpulkan bahwa senyawa D 1 termasuk golongan terpenoid. Dari hasil pengujian sitotoksik ekstrak etil asetat didapatkan nilai LC 50 sebesar 3,68 ppm dan dapat dinyatakan bahwa ekstrak etil asetat kulit batang meranti rambai memiliki sifat sangat toksik terhadap kematian Arte mia salina Leach.Kata kunci: meranti rambai; Shorea acuminata Dyer; terpenoid; sitotoksik; BSLT (BSLT). Isolation was performed by column chromatography using gradient system and then compound of D 1 was obtained in the form of a white amorphous as much as 18 mg with a melting point of 280282°C . Based on the results of the reaction with the LiebermannBuchard reagent (ie brownish orange color) and characterization by UV spectroscopy, IR, 1 H NMR, and 13 CNMR , it can be concluded that the compound of D 1 belonged to the terpenoids group. From the cytotoxicity test, it was found that ethyl acetate extract had LC 50 values of 3.68 ppm and can be stated that the ethyl acetate extract of bark of meranti rambai is very toxic to the Artemia salina Leach. ABSTRACT: Isolation and cytotoxicity test of ethyl acetate extract of the bark of Shorea acuminata Dyer (meranti rambai) and have been done. Extraction was carried out by gradually maceration and cytotoxi city test by Brine Shrimp Lethality Test
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