ABSTRACT:The objective of this research is to compare and evaluate the methane production from cheese whey in single-stage and two-stage anaerobic treatment processes. Single stage-reactor was operated in a pilot-scale batch reactor while two-stage reactor was operated in batch-dilution and semi-continuously. Reactors were loaded with cheese whey with an chemical oxygen demand (COD) of about 38.61 g/L. Single stage system was operated at 35°C with a hydraulic retention time (HRT) of 15, 10, 7 and 4 days; respectively. The maximum methane production in the single-stage reactor was obtained at 3.86 gCOD/L.d organic loading rate (OLR) with 10 days HRT being about 0.39 L CH4 /Lreactor/g CODdeg.d; respectively. The results indicated that the use of a two-stage process for anaerobic treatment of cheese whey resulted in 4 fold higher methane yield compared to single-stage reactor system allowing better control of the acidogenic and methanogenic phases. Keywords: Anaerobic treatment, Biogas, Cheese whey, Hydraulic retention time, Methane production, Phase separationPeyniraltı Suyu Arıtımında Tek ve Çift Aşamalı Anaerobik Reaktörün Optimizasyonu ÖZET: Bu çalışmanın amacı tek ve çift aşamalı anaerobik arıtım proseslerinde peyniraltı suyunda metan üretimini araştırmak ve karşılaştırmaktır. Tek aşamalı reaktör pilot ölçekli kesikli reaktörde işletilirken, çift aşamalı reaktör yarı sürekli ve kesikli olarak işletilmiştir. Reaktör, 38,61g/L kimyasal oksijen ihtiyacına (KOİ) sahip peyniraltı suyu ile yüklenmiştir. Tek aşamalı sistem 15, 10, 7 ve 4 günlük HRT'ler ile 35 0 C'de işletilmiştir ve maksimum metan üretimi HRT'nin 10 gün olduğu işletme koşulunda 3,86 gKOİ/L.gün seviyelerine ulaşmıştır (0,39 L CH4 /Lreaktör/g KOİdeg.gün). Çalışma sonuçları peyniraltı suyunun anaerobik arıtımında asidojenik ve metanojenik fazların daha iyi kontrol altına alınabildiği iki aşamalı prosesin tek aşamalı prosese göre 4 kat daha fazla metan üretim verimi elde edilebildiğini göstermiştir.
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