Especially in the last decade, a vast number of papers on Se and its role in health issues have been published. This review gives a brief, critical overview of the main analytical findings reported in these papers. Of particular interest is the Se content in different food sources worldwide and the extent to which their consumption is reflected in the Se content of human tissues and body fluids. Several food sources, both natural (Brazil nuts, garlic, Brassica juncea) and Se-enriched (yeast-based supplements), are discussed as to origin, characteristics, Se metabolism and impact of their consumption on the human body. The continuous development of new and improvement of existing analytical techniques has provided different powerful tools to unravel the Se species and their function. An up-to-date literature study on Se speciation analysis is given, illustrating how analytical chemistry in its different facets aids in the identification of Se compounds and provides insight into the complete metabolic pathway of Se throughout the human body. This review includes a detailed image of the current state-of-the-art of Se speciation analysis in these food sources and in human tissues and body fluids.
An LC-MS based method for the profiling and characterization of ceramide species in the upper layer of human skin is described. Ceramide samples, collected by tape stripping of human skin, were analyzed by reversed-phase liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry operated in both positive and negative electrospray ionization mode. All known classes of ceramides could be measured in a repeatable manner. Furthermore, the data set showed several undiscovered ceramides, including a class with four hydroxyl functionalities in its sphingoid base. High-resolution MS/MS fragmentation spectra revealed that each identified ceramide species is composed of several skeletal isomers due to variation in carbon length of the respective sphingoid bases and fatty acyl building blocks. The resulting variety in skeletal isomers has not been previously demonstrated. It is estimated that over 1000 unique ceramide structures could be elucidated in human stratum corneum. Ceramide species with an even and odd number of carbon atoms in both chains were detected in all ceramide classes. Acid hydrolysis of the ceramides, followed by LC-MS analysis of the end-products, confirmed the observed distribution of both sphingoid bases and fatty acyl groups in skin ceramides. The study resulted in an accurate mass retention time library for targeted profiling of skin ceramides. It is furthermore demonstrated that targeted data processing results in an improved repeatability versus untargeted data processing (72.92% versus 62.12% of species display an RSD < 15%).
Rising population
density and global mobility are among the reasons
why pathogens such as SARS-CoV-2, the virus that causes COVID-19,
spread so rapidly across the globe. The policy response to such pandemics
will always have to include accurate monitoring of the spread, as
this provides one of the few alternatives to total lockdown. However,
COVID-19 diagnosis is currently performed almost exclusively by reverse
transcription polymerase chain reaction (RT-PCR). Although this is
efficient, automatable, and acceptably cheap, reliance on one type
of technology comes with serious caveats, as illustrated by recurring
reagent and test shortages. We therefore developed an alternative
diagnostic test that detects proteolytically digested SARS-CoV-2 proteins
using mass spectrometry (MS). We established the Cov-MS consortium,
consisting of 15 academic laboratories and several industrial partners
to increase applicability, accessibility, sensitivity, and robustness
of this kind of SARS-CoV-2 detection. This, in turn, gave rise to
the Cov-MS Digital Incubator that allows other laboratories to join
the effort, navigate, and share their optimizations and translate
the assay into their clinic. As this test relies on viral proteins
instead of RNA, it provides an orthogonal and complementary approach
to RT-PCR using other reagents that are relatively inexpensive and
widely available, as well as orthogonally skilled personnel and different
instruments. Data are available via ProteomeXchange with identifier
PXD022550.
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