RESUMELa drépanocytose est une maladie génétique qui constitue un problème de santé publique en Côte d'Ivoire. Etant donné les coûts onéreux du traitement, les patients s'orientent vers la médecine traditionnelle avec l'utilisation de plantes, parmi lesquelles Cajanus cajan. L'objectif de ce travail était de réaliser une étude phytochimique et d'évaluer in vitro l'effet antifalcimiant des graines de Cajanus cajan. Cette étude expérimentale s'est effectuée en deux phases : une première consacrée à la caractérisation des graines et l'autre concernait l'étude de l'activité antifalcimiante in vitro de ces graines. L'étude de l'activité antifalcimiante s'est faite sur les prélèvements sanguins de 30 drépanocytaires homozygotes SSFA 2 . Après induction de la falciformation des globules rouges, l'ajout de l'extrait aqueux de la plante a permis d'évaluer l'activité antifalcimiante par la recherche des drépanocytes au microscope optique. Les Characterization and in vitro evaluation of the antisickling effect of the seeds of Cajanus cajan (Fabaceae) on sickle cells in Abidjan -Côte d'Ivoire ABSTRACTSickle cell anemia is a genetic disease that constitutes a public health problem in Côte d'Ivoire. Given the high cost of treatment, patients in Africa turn to traditional medicine with the use of plants, including E. N' DRAMAN-DONOU et al. / Int. J. Biol. Chem. Sci. 9(5): 2300-2308, 2015 2301Cajanus cajan. The objective of this work was to make a phytochemical study and to evaluate in vitro the antisickling effect of seeds of Cajanus cajan. This experimental study was carried out in two phases: the first was devoted to the characterization of the seeds and the other concerned the study of the in vitro antisickling activity of these seeds. The study was done on the blood samples of 30 SSFA 2 homozygote patients with sickle cell anemia. After induction of the sickling of red blood cells, the addition of the aqueous extract of the plant allowed the evaluation of the antisickling activity by the search for sickle cells under an optical microscope. Cajanus cajan seeds contain sterols, polyterpenes, polyphenols, flavonoids, tannins and alkaloids. They are without any acute toxicity. The aqueous extract reduced the rate of sickle cells of about 50% after 30 min of contact. This study made it possible to show that the seeds of Cajanus cajan have antisickling virtues.
Abstract Aim : Type 3 von Willebrand disease (VWD) is the most severe form of VWD, characterized by a near-total absence of von Willebrand factor (vWF) leading to a huge deficiency in plasmatic factor VIII (FVIII). VWD may be confused with hemophilia A, sometimes leading to misdiagnosis. The purpose of this work was to finalize the biological diagnosis of patients with FVIII activity deficiency in Abidjan, in order to guide the best type of management. Methods: We conducted a cross-sectional descriptive study from June 2018 to April 2019. Forty-nine patients, all of whom had lower FVIII levels or had been referred for bleeding disorder, were monitored in the clinical hematology service. Pro-coagulant activity of coagulation factors was performed in Abidjan. The assays for von Willebrand antigen and activity were performed at Nîmes University Hospital in France. Results: The mean age of patients was 13.8 years (1 – 65) and 86% were Ivorian. FVIII deficiency was discovered during a biological checkup, circumcision or post-traumatic bleeding, in 33%, 31% and 29% respectively. The FVIII level of patients was classified as severe (89.8%), moderate (8.2%) and mild (2%). Only one patient had a quantitative deficiency of von Willebrand factor (vWF: Ag <3%) with undetectable von Willebrand factor activity (vWF: Ac) and an FVIII level <1%. Conclusion: Not all of the constitutive deficits of FVIII are hemophilia A. It was very important to assess the Willebrand factor of these patients followed in Côte d'Ivoire for whom hemophilia A had been suspected.
; Tel : +225 05 31 28 13 RESUME La période périnatale est propice à l'apparition ou à l'aggravation de nombreuses pathologies dépressives susceptibles de retentir sur le déroulement de la grossesse et le développement de l'enfant. Plusieurs mécanismes biologiques et psychosociaux peuvent affecter l'enfant et la mère. L'objectif de ce travail était de décrire le profil hématologique du nouveau-né et de sa mère au cours de la dépression anténatale. L'état mental des mères a été évalué par le Patient Heath Questionnaire module (PHQ9). Le matériel biologique était constitué du sang de cordon ombilical des nouveau-nés et du sang veineux des mères sur lesquels la numération formule sanguine a été réalisé. L'anémie était présente chez 64,1% des déprimées de type hypochrome microcytaire et de 56,3% des non déprimées. L'hyperleucocytose a été observé chez les déprimées (9,4%) et les non déprimées (7,8%). La thrombopénie et la thrombocytose ont été observées indépendamment de l'état mental, 56% des nouveau-nés étaient anémiés. Une différence statistiquement significative a été observé pour le taux de lymphocytes avec un p=0,033. La thrombopénie et la thrombocytose étaient présentes chez les nouveau-nés indépendamment de l'état mental des mères. Le profil hématologique des mères et des nouveau-nés présente quelques particularités qui méritent d'être explorées. © 2016 International Formulae Group. All rights reserved.Mots clés : Dépression anténatale, Hémogramme, Nouveau-né, femmes enceintes, Abidjan. Hematological parameters during antenatal depression ABSTRACTThe perinatal period is favorable to the emergence or aggravating of many depressive pathologies which may have repercussions in the course of pregnancy and child development. Several biological and psychosocial mechanisms can affect the child and the mother. The purpose of this study was to describe the hematological profile of the newborn and his mother during the Depression. Mental status of mothers was evaluated by the PHQ9. The biological material consisted of the umbilical cord blood of newborns and mothers' venous blood in which the blood count was performed. Anemia was present in 64.1% of depressed hypochromic microcytic type and 56.3% of non-depressed. Leukocytosis was observed in both depressed A.R. N'GUESSAN-BLAO et al. / Int. J. Biol. Chem. Sci. 10(6): 2423-2434 2424 (9.4%) and non-depressed (7.8%). thrombocytopenia and thrombocytosis were observed regardless of the mental state, 56% of newborns were anemic. A statistically significant difference was observed for the cell count with p = 0.033. Thrombocytopenia and thrombocytosis were present in newborns regardless of the mental state of mothers. The hematological profile of mothers and newborns has some features that worth exploration
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