Biotransformations of epiandrosterone (1), dehydroepiandrosterone (2), testosterone (3), progesterone (4) and pregnenolone (5) by Aspergillus tamarii MRC 72400 for 5 days have been reported and the results of these incubations have been compared with previously published data obtained with Aspergillus tamarii QM 1223. A. tamarii MRC 72400 showed higher Bayer-Villiger monooxygenase activities than A. tamarii QM 1223 did. Apart from pregnenolone (5), A. tamarii MRC 72400 metabolized these steroids in different ways. Incubation of epiandrosterone (1) afforded 3β,11β-dihydroxy-5α-androstan-17-one (6) (3%) and 3β-hydroxy-17a-oxa-D-homo-5α-androstan-17-one (7) (9.5%). Incubation of dehydroepiandrosterone (2) afforded 3β-hydroxy-17a-oxa-D-homoandrost-5-en-17-one (8) (28%), testolactone (9) (6%), 3β,7β-dihydroxyandrost-5-en-17-one (10) (13%) and 3β,7α-dihydroxyandrost-5-en-17-one (11) (24%). Incubation of testosterone (3) afforded testolactone (9) (58%). Incubation of progesterone (4) also afforded testolactone (9), however in higher yield (86%). Incubation of pregnenolone (5) afforded 3β-hydroxy-17a-oxa-D-homoandrost-5-en-17-one (8) (25%) and testolactone (9) (27%).A number of investigations involving microbial biotransformations of a wide range of steroidal substrates have been carried out due to their potential for the preparation of more valuable and functionalized compounds such as steroid drugs and hormones 1-3 . There are still enormous efforts to increase the efficiency of microbial steroid biotransformations and to find new useful microorganisms and reactions 1 . For example, some of these steroid biotransformations have been carried out using Aspergillus wentii 4 , Penicillium digitatum 5 and Aspergillus terreus 6 . A. wentii showed some high hydroxylase activities whereas P. digitatum and A. terreus showed some low 5α-reductase and Baeyer-Villiger monooxygenase (BVMO) activities, respectively.
The biotransformations of testosterone, epiandrosterone, progesterone and pregnenolone byAspergillus terreusMRC 200365 for five days were described. The biotransformation of testosterone afforded testolactone. The biotransformation of epiandrosterone afforded 3β-hydroxy-17a-oxa-D-homo-5α-androstan-17-one. The biotransformation of progesterone afforded androst-4-ene-3,17-dione and testolactone. The biotransformation of pregnenolone afforded 3β-hydroxy-17a-oxa-D-homoandrost-5-en-17-one and testolactone.
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