Listeria je patogen prenosiv hranom i predstavlja veliku opasnost za ljudsko zdravlje. Teški su simptomi praćeni visokom stopom mortaliteta većinom prouzročeni L. monocytogenes. Bez obzira na politiku nulte tolerancije, rod Listeria je još uvijek prisutan u sirovim proizvodima i proizvodima spremnim za konzumiranje, predstavljajući veliku opasnost za zdravlje ljudi. Za sada još u bliskoj budućnosti ne postoje studije o kontaminaciji hrane vrstama Listeria u Bosni i Hercegovini. Ovim istraživanjem htjeli smo dobiti osnovne podatke o količini kontaminiranosti hrane ovim patogenom. U studiji je analizirano ukupno 238 uzoraka hrane, pri čemu su u 105 uzorka bile različite vrste sirove hrane te 133 uzorka hrane spremne za konzumaciju (gotove hrane). Od ukupno 238 uzoraka, 18 uzoraka je bilo pozitivno na Listeria vrste. Nakon izolacije i detekcije, konfirmacija pozitivnih izolata Listeria spp. je provedena pomoću biokemijskog kita API® Listeria.
Meat inspection is an important part of education for every veterinary student. However, traditional teaching methods require the sacrifice of living animals, and are thus considered expensive, inadequate and inhumane. Development of novel technologies has provided opportunities for new, improved ways of education. Smart 3D Meat Inspection (S3DMI) is an elearning tool that allows veterinary medicine students to acquire required skills using virtual 3D models of animal organs and carcasses. These models can be manipulated and “cut” just like real organs, allowing students to learn this essential skill without the need for animal carcasses. Students are allowed to practice any part of meat inspection as many times necessary, at their own pace, without time, place or resources limitations. This type of education is considered superior to traditional methods. There is no need for sacrification of animals for educational purposes and the cost of education is greatly reduced, while the educational quality is uninterrupted. Models developed for S3DMI can also be adjusted for courses like animal anatomy and pathology, which also require the use of real animal cadavers. S3DMI is still in its developmental stages, but it has a great potential to minimalize the need for animal sacrifice in the education of future veterinarians, while ensuring the quality improvement.
The aim of this research was to examine physicochemical parameters of finished and semi-finished meat products: minced meat (beef, pork and mixed meat) and raw formed meat products (ćevapi, patties/burgers and sujuk sausages), and to determine quality parameters of these products on the Bosnia and Herzegovina market. Examined parameters included: protein content, fat, collagen in meat proteins and salt (NaCl). We analysed 282 samples of these products and found deviations with regard to the applicable Ordinance on minced meat and semi-finished and finished meat products of “The Law on Food of Bosnia and Herzegovina” in all parameters, except salt (NaCl). The majority of deviations referred to the content of collagen in meat proteins, with as much as 21.98 % of examined samples in violation of the applicable legislation.
The objective of this paper was to create an economical and abecedarian 3D printing method for the production of solid microneedles, as a more efficient transdermal drug delivery method, for day-to-day use in companion animals. The process of 3D printing was conducted using two types of 3D printers, utilizing the FDM and SLA printing techniques. Modulus of Elasticity was calculated for the determination of mechanical properties of the material, wherethe printed specimen was subjected to axial loading, and deformations were measured using an optical scanner. Post-processing was conducted by washing microneedles in isopropyl alcohol, followed by UV curing. The procedure of testing penetration capabilities was conducted at two sites of cat skin: Auris externa and the lateral part of the abdomen. The SLA printing method was more precise, resulting in higher quality microneedles for animal use compared to the FDM printing technique. Modulus of Elasticity was calculated and the value E=0.9 GPa can be used. Testing proved that the printed model was able to penetrate the skin at the tested sites. The use of microneedles is simple and economical, and therefore has wide applications in small animal practice. Veterinarians can access microneedle design repositories and print them for more effective transdermal drug delivery. The multifunctionality and transferability of the design in the present study ensure that it can be further modified to provide personalized therapy.
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