The main purpose of this research was to examine the construct validity of the Depression, Anxiety and Stress Scales (DASS-21) in order to determine whether it is able to adequately discriminate between symptoms of depression and anxiety in the Hispanic population in Puerto Rico. This study has an instrumental design. A total of 1073 Hispanics participated in this psychometric study. The results showed that the DASS-21 has serious psychometric deficiencies, especially related to the construct validity, as well as convergent and discriminatory validity. In addition, it was shown that DASS-21 do not replicate the three-dimensional structure of the original instrument in the Hispanic community. Finally, it was confirmed that the DASS-21 have difficulty in properly identifying and discriminating between symptoms associated with depression and anxiety in a Hispanic population.

The purpose of this online study was to develop an explicative model regarding the origin of infidelity-related behaviors on social networks for Hispanic women. We propose that sexual satisfaction and emotional intimacy have a direct impact on the satisfaction of couple relationships, and an indirect impact in the development of infidelity-related behaviors on social networks. To investigate this proposal, we used a non-probabilistic sample of 341 Hispanic women living in Puerto Rico. Statistical analyses confirmed that satisfaction and ambivalence in couple relationship completely mediate the association between sexual satisfaction and infidelity-related behaviors on social networks, as well as the relationship between emotional intimacy and infidelity-related behaviors on social networks. Overall, women who practice infidelity-related behaviors on social networks showed less sexual satisfaction, less emotional intimacy, less relationship satisfaction, and greater ambivalence. Our results provide theoretical and empirical evidence on how infidelity-related behaviors on social networks develop in couple relationships, and these results could help to inform possible forms of prevention and intervention.

Acute exposure to morphine after a traumatic event reduces trauma related symptoms in humans and conditioned fear expression in male rats. We aimed to determine whether acute administration of morphine alters consolidation of fear learning and extinction. Male and female rats in proestrus and metaestrus (high and low ovarian hormones respectively) underwent fear conditioning and received saline or morphine (2.5 mg/kg s.c.). The next day they underwent extinction. Results showed increased freezing during extinction only in the morphine metaestrus group while morphine did not affect males or proestrus females. Recall of extinction was similar on all groups. On a second experiment, a subset of rats conditioned during metaestrus was administered morphine prior to extinction producing no effects. We then measured mu opioid receptor (MOR) expression in the amygdala and periaqueductal gray (PAG) at the end of extinction (day 2). In males and proestrus females, morphine caused an increase in MOR in the amygdala but no in the PAG. In metaestrus females, morphine did not change MOR expression in either structure. These data suggests that ovarian hormones may interact with MORs in the amygdala to transiently alter memory consolidation. Morphine given after trauma to females with low ovarian hormones might increase the recall of fear responses, making recovery harder.

The mu opioid receptor (MOR) has a significant role in fear conditioning and extinction learning. Communication between amygdala, periaqueductal gray (PAG) and medial prefrontal cortex (mPFC) regulates fear conditioning and extinction. Fear conditioned female rats during the metaestrus (M, low hormones) stage of the estrous cycle, showed impaired extinction while rats during the proestrus (P, high hormones) stage showed normal extinction learning. We aim to elucidate how opioid modulators alter MOR expression during fear conditioning and extinction in both sexes. Female rats on M or P stages and males rats underwent auditory fear conditioning and immediately received MOR agonist morphine, or antagonist naloxone or saline. The next day rats received extinction trials. Western blots were used to measure MOR expression. In the PAG, female rats showed no change with either treatment. In male rats morphine produced an increase in MOR expression while naloxone had an opposite effect. In the PFC, morphine during P decreased MOR expression while naloxone had no effect. Increased MOR expression was observed in the amygdala of male rats after either morphine or naloxone treatments, while in females an increase in MOR was only observed during P. Our data portraits sex differences in MOR expression in the fear circuitry, which might be responsible for the persistence of fear responses during low ovarian hormone stages. Grant Funding Source: Supported by Molecular and Genomics core RR003050, Behavioral Core Facilities MD007579 , HPD335698 Nova Southeastern University to Edwin Santini and Dinah Ramos‐Ortolaza

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