The objective of this study was to characterize the relationship between rumination and subclinical ketosis (SCK) in transition dairy cows. A study was conducted on 4 commercial dairy farms in eastern Ontario, Canada. A total of 339 Holstein dairy cows (107 primiparous and 232 multiparous) were monitored for rumination activity and SCK from 14 d before calving until 28 d after calving. Rumination was recorded daily using an automated monitoring system. A blood sample was taken from the coccygeal vein of each cow for measurement of β-hydroxybutyrate (BHB) once weekly throughout the 6-wk observation period. Cows with BHB ≥1.2mmol/L in any of the 4 postpartum samples were considered to have SCK. Cases of retained placenta, metritis, milk fever, or mastitis during the study period were also recorded. Cows were categorized into 1 of 4 groups: healthy cows (HLT) that had no SCK or any other recorded health problem (n=139); cows treated for at least one health issue other than SCK (HLT+; n=50); cows with SCK (hyperketonemia; HYK) with no other health problems during transition (n=97); or cows (HYK+) that had SCK and one or more other health problems (n=53). Daily rumination time was summarized by week and comparisons were made between HLT and HYK and HYK+. From 2 wk before calving (wk -2) to 4 wk after calving (wk +4), there was no difference in rumination time (409±9.8min/d) among HLT, HYK, and HYK+ cows in their first lactation. Multiparous cows in HLT spent an average of 459±11.3min/d ruminating from wk -2 to wk +4. Multiparous HYK cows ruminated 25±12.8min/d less than HLT cows, whereas HYK+ cows ruminated 44±15.6min/d less than HLT cows. The largest differences in rumination time between HLT and HYK+ cows were seen during wk -1, +1, and +2, when HYK+ cows ruminated 48±17.2, 73±16.0, and 65±19.4min/d less than HLT cows, respectively. In multiparous cows, increased odds of HYK were associated with greater milk yield in the previous lactation, greater loss of body condition over the transition period, greater stall stocking density in wk -1, and reduced rumination time in wk -1. Increased odds of HYK+ were associated with higher parity, longer dry period, greater stall stocking density in wk -1, and reduced rumination time in wk +1. These results suggest that rumination monitoring across the transition period might contribute to identification of SCK and other health problems in multiparous cows.

The objective of this study was to characterize the association of lying behavior and subclinical ketosis (SCK) in transition dairy cows. A total of 339 dairy cows (107 primiparous and 232 multiparous) on 4 commercial dairy farms were monitored for lying behavior and SCK from 14d before calving until 28 d after calving. Lying time, frequency of lying bouts, and average lying bout length were measured using automated data loggers 24h/d. Cows were tested for SCK 1×/wk by taking a blood sample and analyzing for β-hydroxybutyrate; cows with β-hydroxybutyrate ≥1.2mmol/L postpartum were considered to have SCK. Cases of retained placenta, metritis, milk fever, or mastitis during the study period were recorded and cows were categorized into 1 of 4 groups: healthy (HLT) cows had no SCK or any other health problem (n=139); cows treated for at least 1 health issue other than SCK (n=50); SCK (HYK) cows with no other health problems during transition (n=97); or subclinically ketotic plus (HYK+) cows that had SCK and 1 or more other health problems (n=53). Daily lying time was summarized by week and comparisons were made between HLT, HYK, and HYK+, respectively. We found no difference among health categories in lying time, bout frequency, or bout length fromwk -2 towk +4 relative to calving for first-lactation cows. Differences in lying time for multiparous cows were seen inwk +1, when HYK+ cows spent 92±24.0 min/d more time lying down than HLT cows, and duringwk +3 and +4 when HYK cows spent 44±16.7 and 41±18.9 min/d, respectively, more time lying down than HLT cows. Increased odds of HYK+ were found to be associated with higher parity, longer dry period, and greater stall stocking density inwk -1 and longer lying time duringwk +1. When comparing HYK to HLT cows, the same variables were associated with odds of SCK; however, lying time was not retained in the final model. These results suggest that monitoring lying time may contribute to identifying multiparous cows experiencing SCK with another health problem after calving, but may not be useful in the early detection of SCK.

The objective of this study was to determine the associations of rumination time (RT) and health status with milk yield and milk composition. This study used 339 dairy cows from 4 commercial dairy farms in Ontario, Canada (first lactation, n = 107; second lactation, n = 112; ≥third lactation, n = 120). Rumination time was monitored (24 h/d) using an automated system from 1 to 28 d in milk (DIM). Cows were milked 3×/d on each farm, and 2 farms recorded milk weights at each milking to determine daily milk yield (n = 170). Cows were also monitored for milk composition (fat and protein content) 1×/wk. Last, subclinical ketosis (SCK) was diagnosed 1×/wk; cows with at least one blood sample with β-hydroxybutyrate ≥1.2 mmol/L postcalving were diagnosed with SCK. Cases of retained placenta, metritis, milk fever, or mastitis during the study period were also recorded. Cows were categorized into 1 of 4 groups: healthy cows that had no SCK or any other health issue (HLT; n = 139); cows that were treated for at least one health issue other than SCK (HLT+; n = 50); SCK cows with no other health problems during transition (HYK; n = 97); or cows that had SCK and one or more other health problems (HYK+; n = 53). All data were summarized by week across cows, and the associations between rumination time and milk yield (n = 170) and milk composition (n = 339) were modeled. Across all lactations, and including all health categories, milk yield increased by week, whereas fat and protein content both decreased by week. A positive association was found between summarized RT and milk yield in first-lactation (+0.006 ± 0.003 kg/min of RT) and second-lactation (+0.015 ± 0.004 kg/min of RT) cows from 4 to 28 DIM, as well as in ≥third-lactation cows; however, the relationship between RT and milk yield differed across weeks in those cows. A negative association between RT and milk fat content was found in ≥third-lactation cows (-0.002 ± 0.00059 percentage points/min of RT). From 4 to 28 DIM, ≥third-lactation HYK and HYK+ cows produced less protein (0.11 ± 0.051 and 0.13 ± 0.056 percentage points, respectively) than HLT cows. Over the 4-wk observation period, first-lactation HYK+ cows tended to deposit 0.11 ± 0.056 percentage points less protein in their milk compared with HLT cows. Second-lactation HYK+ cows produced less milk than HLT cows each week during early lactation. In summary, RT was positively associated with milk yield in early-lactation dairy cows, across all lactations, and negatively associated with milk fat content in ≥third-lactation cows. Further, the results showed that early-lactation cows that experience SCK, particularly with one or more other health problems, might have decreased milk yield and milk protein content.

Tangled active filaments are ubiquitous in nature, from chromosomal DNA and cilia carpets to root networks and worm collectives. How activity and elasticity facilitate collective topological transformations in living tangled matter is not well understood. We studied California blackworms ( Lumbriculus variegatus ), which slowly form tangles in minutes but can untangle in milliseconds. Combining ultrasound imaging, theoretical analysis, and simulations, we developed and validated a mechanistic model that explains how the kinematics of individual active filaments determines their emergent collective topological dynamics. The model reveals that resonantly alternating helical waves enable both tangle formation and ultrafast untangling. By identifying generic dynamical principles of topological self-transformations, our results can provide guidance for designing classes of topologically tunable active materials.

Many organisms utilize group aggregation as a method for survival. The freshwater oligochaete, Lumbriculus variegatus (California blackworms) form tightly entangled structures, or worm “blobs”, that have adapted to survive in extremely low levels of dissolved oxygen (DO). Individual blackworms adapt to hypoxic environments through respiration via their mucous body wall and posterior ciliated hindgut, which they wave above them. However, the change in collective behavior at different levels of DO is not known. Using a closed-loop respirometer with flow, we discover that the relative tail reaching activity flux in low DO is ∼75x higher than in the high-DO condition. Additionally, when flow rate is increased to suspend the worm blobs upward, we find that the average exposed surface area of a blob in low DO is ∼1.4x higher than in high DO. Furthermore, we observe emergent properties that arise when a worm blob is exposed to extreme DO levels. We demonstrate that internal mechanical stress is generated when worm blobs are exposed to high DO levels, allowing them to be physically lifted off from the bottom of a conical container using a serrated endpiece. Our results demonstrate how both collective behavior and the emergent generation of internal mechanical stress in worm blobs change to accommodate differing levels of oxygen. From an engineering perspective, this could be used to model and simulate swarm robots, self-assembly structures, or soft material entanglements.