Estuarine food webs are fueled by multiple different primary producers. However, identifying the relative importance of each producer to consumers is difficult, particularly for fishes that utilize multiple food sources due to both their mobility and their generally high trophic levels. Previous studies have documented broad spatial differences in the importance of primary producers to fishes within the Upper San Francisco Estuary, California, including separation between pelagic and littoral food webs. In this study, we evaluated the importance of primary producers to adult fishes in three closely spaced subregions that represented disparate habitat types (a tidal wetland channel, a turbid backwater channel, and a deep open-water channel), each a potential outcome of local restoration projects. Using stable isotope analysis coupled with a Bayesian mixing model, we identified significant differences in primary-producer contribution to fishes and invertebrates across habitats and seasons, especially in the relative contribution of submersed aquatic vegetation and phytoplankton. Most fishes utilized multiple primary producers and showed little segregation between pelagic and littoral food webs among habitats. Availability of primary producers differs seasonally and across multiple spatial scales, helping to buffer environmental variability and thus enhancing food web resilience. Ecosystem restoration may improve with emphasis on restoring a wide variety of primary producers to support consumers.

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We compared the extent to which ancient and restoring wetlands in three estuary regions of San Francisco Bay support estuarine ecosystems through food web contributions. In comparison to mature marshes, we hypothesized that food webs of increasingly younger restoration sites would display increased dependency upon allochthonous subsidies due to nominal internal production. Using multiple stable isotopes (δ 13 C, δ 15 N, δ 34 S) in a mixing model, we traced links among primary producers and estuarine consumers. Results indicate that food webs of estuarine marshes are heavily dependent upon autochthonous marsh materials (76±17%), even within the youngest restoration marshes (11 years). Nearly all sampled organisms relied upon autochthonous marsh materials, with the exception of Neomysis kadiakensis, a mysid shrimp, which derived the majority of its support from freshwaterproduced phytoplankton. Marsh-derived organic matter (OM) support was consistent both temporally throughout the year and spatially along the three estuary regions, but evidence suggests that the specific type of OM supporting estuarine consumers depends on position along the estuarine gradient and on seasonal shifts in freshwater flow. These results indicate that wetland restoration rapidly provides important contributions to marsh consumers and potentially bolsters food web linkages in shallow-water ecosystems.

Tidal marsh wetlands provide important foraging habitat for a variety of estuarine fishes. Prey organisms include benthic/epibenthic macroinvertebrates, neustonic arthropods, and zooplankton. Little is known about the abundance and distribution of interior marsh macroinvertebrate communities in the San Francisco Estuary (estuary). We describe seasonal, regional, and site variation in the composition and abundance of neuston and benthic/epibenthic macroinvertebrates that inhabit tidal marsh channels, and relate these patterns to environmental conditions. We also describe spatial and temporal variation in diets of marsh-associated inland silverside, yellowfin goby, and western mosquitofish. Fish and invertebrates were sampled quarterly from October 2003 to June 2005 at six marsh sites located in three river systems of the northern estuary: Petaluma River, Napa River, and the west Delta. Benthic/epibenthic macroinvertebrates and neuston responded to environmental variables related to seasonal changes (i.e., temperature, salinity), as well as those related to marsh structure (i.e., vegetation, channel edge). The greatest variation in abundance occurred seasonally for neuston and spatially for benthic/epibenthic organisms, suggesting that each community responds to different environmental drivers. Benthic/epibenthic invertebrate abundance and diversity was lowest in the west Delta, and increased with increasing salinity. Insect abundance increased during the spring and summer, while Collembolan (springtail) abundance increased during the winter. Benthic/epibenthic macroinvertebrates dominated fish diets, supplemented by insects, with zooplankton playing a minor role. Diet compositions of the three fish species overlapped considerably, with strong selection indicated for epibenthic crustaceans-a surprising result given the typical classification of Menidia beryllina as a planktivore, Acanthogobius flavimanus as a benthic predator, and Gambusia affinis as a larvivorous surface-feeder. Fish diets were influenced by position along the estuarine gradient and season. Overall, our data show that local-scale site effects and marsh position within the estuary influence invertebrate community composition and abundance. Additionally, we show that restoring marsh ecosystems can subsidize fishes similarly to reference marshes. We thus recommend that managers focus on the ability of restoring marshes to produce food subsidies for target species when planning and designing tidal marsh restoration projects, especially those targeted for food web support.

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We measured δ 13 C, δ 15 N and δ 34 S signatures of natural and translocated mussels Ischadium demissum to identify food web source differences among estuarine marshes displaying various stages of restorative development. We hypothesized that mussels inhabiting younger marshes would be more dependent on allochthonous organic matter sources, while those inhabiting mature marshes would depend on autochthonous sources. Mussels collected from an undisturbed (reference) marsh located within the Napa River estuarine complex in San Francisco Bay were translocated to a series of restoring marsh sites located within the same river system. The isotopic composition of naturally growing mussels was compared with translocated mussels, which were incubated in restoring sites for 5 and 7 mo. Measurements of δ 13 C, δ 15 N, and δ 34 S indicated differences in food web sources supporting I. demissum among the 4 marsh sites. A strong cage effect was detected during the initial 5 mo collection interval, indicating that translocated mussels had yet to equilibrate with their new environments. Multiple source mixing model analysis indicated that C 3 emergent vascular plants and brackish phytoplankton contributed most of the organic matter consumed by I. demissum over both time periods, but that mussels collected from the downstream sites exhibited higher dependence upon vascular plant detritus. Bay produced phytoplankton contributed very little to I. demissum diets, suggesting that the pelagic waters of San Francisco Bay have less influence on marsh food web dynamics than previously anticipated. The results of this experiment show that food web pathways are strongest at intermediate scales; they can be relatively short and unique to specific marshes along the estuarine gradient, but similarities in mussel diets among marshes in close proximity to one another suggests inter-marsh exchange of organic matter. It is, therefore, likely that food webs in young restoration sites depend upon organic matter subsidies from neighboring marshes, rather than from San Francisco Bay. KEY WORDS: Restoration · Mussels · Ischadium demissum · Food web · Estuarine marshes · San Francisco Bay Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 351: [65][66][67][68][69][70][71][72][73][74][75][76] 2007 ocean (Odum 2000). A primary goal of estuarine marsh restoration is to recover those ecological functions, such as cross boundary energy subsidies, which enhance coastal production of fish and wildlife (Kneib 2003, McCay et al. 2003. As recently noted by Wozniak et al. (2006), who directly examined carbon flows that run through restoring salt-marsh food webs, the majority of post-restoration studies limit their focus to structural differences between reference and restoring sites and do not address functional trajectories associated with marsh restoration.In contrast to the estuarine outwelling hypothesis (Odum 2000), in which tidal waters export a mix of riverine, marine, terrestrial, and marsh deriv...

In this paper, we focus on 2 mechanisms of cross-boundary food web connectivity in Puget Sound estuaries: passive transport of water-advected organic matter (OM) and active movement of organisms. Both mechanisms serve as potential vectors of food web connectivity, but little research has investigated whether landscape setting changes the dominance of one mechanism over another, or whether the influence of organism movement on food web connectivity can be detected in estuarine systems. We use fish diets, stable isotopes and Bayesian mixing models to identify differences in OM sources assimilated by estuarine fishes, testing whether increased organism mobility or increased fluvial influence results in greater food web connectivity. We compare food web connectivity in 2 different estuaries, one displaying limited freshwater inputs, and the other the terminus of a major river system. Within each estuary, we investigate whether differences in behavioral life history traits correspond to differences in the diets, isotopic signatures and OM assimilation of 2 fish species: bay pipefish Syngnathus leptorhynchus, which displays site fidelity to eelgrass beds, and the more transitory juvenile English sole Parophrys vetulus, which moves throughout estuarine deltas during the early demersal growth stage. Our results show water advection plays a dominant role in large-scale OM transport and delivery to adjoining ecosystems in the fluvial estuary, while organism movement provides the more important mechanism of food web connectivity in the estuary exhibiting minor fluvial discharge. However, the 2 mechanisms certainly interact to enhance food web connectivity across estuarine ecotones.

To evaluate the role of restoration in the recovery of the Delta ecosystem, we need to have clear targets and performance measures that directly assess ecosystem function. Primary production is a crucial ecosystem process, which directly limits the quality and quantity of food available for secondary consumers such as invertebrates and fish.