Conventional methods for rAAV purification that are based vector that is more than 99% pure. More importantly, the on cesium chloride ultracentrifugation have often produced new purification procedures consistently produce rAAV vector preparations of variable quality and resulted in sigstocks with particle-to-infectivity ratios of less than 100, nificant loss of particle infectivity. We report here several which is significantly better than conventional methods. novel purification strategies that involve the use of non-The new protocol increases the overall yield of infectious ionic iodixanol gradients followed by ion exchange or heprAAV by at least 10-fold and allows for the complete purifiarin affinity chromatography by either conventional or cation of rAAV in 1 working day. Several of these methods HPLC columns. These methods result in more than 50%should also be useful for large-scale production. recovery of rAAV from a crude lysate and routinely produce

Mid-century modern architecture developed after the Second World War as numerous technological advancements allowed for open house plans with the increased use of glass and a reconfiguration of indoor-outdoor relationships. Rufus Nims, a Miami architect (1913–2005), hybridized emerging ideas of mid-century modernism with climatic design that emerged in field of tropical architecture after the Second World War. Nims experimented with homes that had disappearing walls; and that could be comfortable in the hot and humid climate of Florida. This paper will analyze Rufus Nims’ role in the development of Florida Tropical Architecture, through his seamless integration of indoor and outdoor spaces. Further, this study will assess how Rufus Nims used tropical architecture strategies in South Florida, such as screened-in porches, disappearing walls, and landscape integration. The paper argues that Rufus Nims’ architectural ideas were based on emerging redefinition of the indoor-outdoor spatial relationships as was evident in the broader mid-century modern movement and Florida Tropical Architecture.