Low-cost, field-deployable, near-time methods for assessing water quality are not available when and where waterborne infection risks are greatest. We describe the development and testing of a novel device for the measurement of tryptophan-like fluorescence (TLF), making use of recent advances in deep-ultraviolet light emitting diodes (UV-LEDs) and sensitive semiconductor photodiodes and photomultipliers. TLF is an emerging indicator of water quality that is associated with members of the coliform group of bacteria and therefore potential fecal contamination. Following the demonstration of close correlation between TLF and E. coli in model waters and proof of principle with sensitivity of 4 CFU/mL for E. coli, we further developed a two-LED flow-through configuration capable of detecting TLF levels corresponding to “high risk” fecal contamination levels (>10 CFU/100 mL). Findings to date suggest that this device represents a scalable solution for remote monitoring of drinking water supplies to identify high-risk drinking water in near-time. Such information can be immediately actionable to reduce risks.

Riparian vegetation restoration efforts demand cost effective, accurate, and replicable impact assessments. In this thesis a method is presented using an Unmanned Aerial Vehicle (UAV) equipped with a GoPro digital camera to collect photogrammetric data of a 2.02-acre riparian restoration. A three-dimensional point cloud was created from the photos using Structure from Motion (SfM) techniques. The point cloud was analyzed and compared to traditional, ground-based monitoring techniques. Ground truth data collected using the status-quo approach was collected on 6.3% of the study site and averaged across the entire site to report stem heights in stems/acre in three height classes, 0-3 feet, 3-7 feet, and greater than 7 feet. The project site was divided into four analysis sections, one for derivation of parameters used in the UAV data analysis, and the remaining three sections reserved for method validation. The most conservative of several methods tested comparing the ground truth data to the UAV generated data produced an overall error of 21.6% and indicated an r 2 value of 0.98. A Bland Altman analysis indicated a 99% probability that the UAV stems/plot result will be within 159 stems/plot of the ground truth data. The ground truth data is reported with an 80% confidence interval of +/-844 stems/plot, thus the UAV was able to estimate stems well within this confidence interval. Further research is required to validate this method longitudinally at this same site and across varying ecologies. These results suggest that UAV derived environmental impact assessments at riparian restoration sites may offer competitive performance and value.

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Riparian vegetation restoration efforts demand cost effective, accurate, and replicable impact assessments. In this thesis a method is presented using an Unmanned Aerial Vehicle (UAV) equipped with a GoPro digital camera to collect photogrammetric data of a 2.02-acre riparian restoration. A three-dimensional point cloud was created from the photos using Structure from Motion (SfM) techniques. The point cloud was analyzed and compared to traditional, ground-based monitoring techniques. Ground truth data collected using the status-quo approach was collected on 6.3% of the study site and averaged across the entire site to report stem heights in stems/acre in three height classes, 0-3 feet, 3-7 feet, and greater than 7 feet. The project site was divided into four analysis sections, one for derivation of parameters used in the UAV data analysis, and the remaining three sections reserved for method validation. The most conservative of several methods tested comparing the ground truth data to the UAV generated data produced an overall error of 21.6% and indicated an r 2 value of 0.98. A Bland Altman analysis indicated a 99% probability that the UAV stems/plot result will be within 159 stems/plot of the ground truth data. The ground truth data is reported with an 80% confidence interval of +/-844 stems/plot, thus the UAV was able to estimate stems well within this confidence interval.Further research is required to validate this method longitudinally at this same site and across varying ecologies. These results suggest that UAV derived environmental impact assessments at riparian restoration sites may offer competitive performance and value.ii Acknowledgements