The present study has been carried out to investigate the possible therapeutic effect of curcumin loaded iron oxide nanoparticles (MNP-CUR) administered through intraperitoneal (i.p) injection after whole body gamma irradiation of rats. Whole body gamma irradiation of rats at 6 Gy (single dose) caused significant reduction in red blood cells count, hemoglobin levels, lymphocytes, neutrophils, and monocytes associated with highly significant elevation in malondialdehyde (MDA). The reduction in catalase (CAT) activity and the reduced levels of glutathione GSH were observed after three weeks postirradiation. Administration of MNP-CUR after irradiation resulted in a significant improvement of the hematological parameters andreduced changes in MDA, GSH and CAT levels were induced by gamma irradiation.The combination of MNP and CUR was found to be advantageous for the after -exposure treatment because the effect of radiation is stealth. An individual may spend days, weeks or even months exposed to radiation doses without becoming alarmed. Exposure to ionizing radiation is known to have an effect on the body cells, especially the blood cells and cause a marked decline in their viability and proliferation.
A B S T R A C TGamma radiation produces numerous biological perturbations in cells by direct ionization of DNA and cellular targets and by indirect effect through damage by free radical production. The present study has been carried out to investigate the possible therapeutic effect of folic acid loaded magnetic nanoparticles through injection after body gamma irradiation of rats. We have shown that the folic acid loaded magnetic nanoparticles administration reduced the risk factors induced by γradiation. 60 male albino rats of about 130-150 g live body weight were divided into six groups, Negative control (n=10), Magnetic control (n=10), folic acid loaded Magnetic NPs control (n=10), and 6 Gy irradiation group (n=10). Three post irradiation groups where these groups exposed firstly to 6 Gy gamma irradiation then injected (I.p) by magnetite NPs, and folic acid loaded magnetic NPs, twice weekly for three weeks. The rats in all groups were anaesthetized, decapitated, the liver was excised immediately, and frozen at -80 0c. DNA damage in rat hepatocytes were determined by comet assay, and determined cholesterol and triglyceride concentration. Results showed that whole body gamma irradiation of rats at 6Gy (single dose) induced significant increase in DNA damage (P < 0.05) that was indicated by increase in tail length, tail DNA% and tail moment as compared to control group. And significant increase in the hepatic cholesterol and triglycerides levels was observed after three weeks post-irradiation Administration of folic acid loaded magnetic iron oxide (Fe3O4) after irradiation induced significant improvement of the DNA damage and hepatic cholesterol and triglyceride level. These results indicate the role of folic acid loaded MNPs as a radio protector agent. Further, we also report the radio protective property of folic acid loaded MNPs as demonstrated by reduction in the radiation induced DNA damage, which was measured by alkaline comet assay.
Gamma radiation produces numerous biological perturbations in cells by direct ionization of DNA and cellular targets and by indirect effect through damage by free radical production. The present study has been carried out to investigate the possible therapeutic effect of folic acid loaded magnetic nanoparticles through injection after whole body gamma irradiation. Results showed that whole body gamma irradiation of rats at 6Gy (single dose) induced a significant increase in DNA damage (P < 0.05) that was indicated by an increase in tail length, tail DNA% and tail moment as compared to control group. Significant increase in the hepatic cholesterol and triglycerides levels was observed after three weeks post-irradiation. Administration of folic acid loaded magnetic iron oxide (Fe3O4) after irradiation induced significant improvement of the DNA damage and hepatic cholesterol and triglyceride level. These results indicate the possible role of folic acid loaded MNPs as a therapeutic agent against the genetic damage caused by ionizing radiation
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