Background: Immune dysregulation is undoubtedly implicated in the pathogenesis of oral lichen planus (OLP) and considering the importance of miR-155 in the regulation of the immune response the present study is conducted to assess salivary levels of miR-155 in patients with oral lichen planus lesions and compare these levels before and after corticosteroid therapy to explore its possible implication in the pathogenesis OLP. Methods: Thirty patients were enrolled in this study, 15 healthy individuals with normal mucosa as controls and 15 patients diagnosed with atrophic/erosive OLP lesions. Patients were treated with topical corticosteroid. Salivary levels of miR-155 were assessed before and after 4 weeks of topical steroids therapy. Clinical improvement was assessed via VAS score and Thongprasom scale. Results: A significant reduction of miR-155 levels four weeks post treatment was recorded with a value of 55.67%. There was a highly significant direct correlation between miR-155 levels and VAS score. Conclusion: The overexpression of miR-155 in OLP patients before treatment with its obvious reduction four weeks after therapy suggests it could be directly involved in OLP pathogenesis and shedding a light on the potential of miR-155 to act as an innovative therapeutic target for OLP.
Background
Rapid diagnosis of acute myocardial infarction (AMI) is the subject of many clinical studies as it enables an effective therapy, preventing adverse progression of AMI and increasing survival rates. Recent studies have revealed that specific blood-based long non-coding RNAs (lncRNAs) are deregulated in patients with AMI and serve as promising diagnostic and prognostic tools. The current study aimed to determine the potential role of a hypoxia-responsive lncRNA, hypoxia-inducible factor 1A antisense RNA 2 (HIF1A-AS2), as a biomarker for early diagnosis and predictor of left ventricular dysfunction (LVD).
Methods
This study was carried out on 48 patients with AMI and 50 age-and sex-matched controls. The relative quantification of HIF1A-AS2 expression was done using reverse transcription real‐time polymerase chain reaction.
Results
Compared to the control group, HIF1A-AS2 were significantly higher in MI patients (P < 0.001). Interestingly, patients presenting within 3 h of chest pain onset had elevated levels of HIF1A‐AS2 as compared to patients with late presentation. The ROC curve was constructed to assess HIF1A-AS2 as an early marker. It demonstrated higher sensitivity (94%) and specificity (86%). Moreover, the multivariate regression analysis revealed that HIF1A‐AS2 was significantly associated with LVD in the patient group after 6 months follow up (p = 0.018).
Conclusion
Our study suggests that HIF1A‐AS2 may be a potential early diagnostic biomarker of AMI with high sensitivity. In addition, it might have a promising role as a predictor of left ventricular dysfunction.
Background:The relation between diabetes mellitus and periodontitis has been discussed for years. Many adipokines were reported to play a major role in periodontal inflammation. Chemerin is one of these adipokines which is blamed to be involved in inflammatory conditions such as diabetes. Fibroblast growth factor 21 (FGF21) is another adipokine which is believed to induce regulation of glucose. The current study aimed to spot the light on the potential role of gingival crevicular fluid (GCF) level of FGF21 and chemerin as biomarkers of periodontal disease activity and an attempt to understand their role in the link between periodontitis and diabetes. Methods: The study was conducted on 45 individuals, 15 diabetic patients with periodontitis, 15 periodontitis patients and 15 healthy controls. GCF samples were collected from all participants for assessment of chemerin and FGF 21. Samples were analysed using enzyme linked immunosorbent assay (ELISA). Results: Periodontitis patients with diabetes had significant higher levels of chemerin and FGF21 than the periodontitis patients without diabetes followed by healthy controls. ROC analysis showed a 100% diagnostic accuracy for chemerin and FGF21. Conclusion: FGF21 and chemerin in GCF are valuable biomarkers of periodontal disease suggesting a role in the link between diabetes and periodontitis.
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