Background: Estrogen deprivation in the postmenopausal women plays a critical role in progression of type 2 diabetes mellitus (T2DM). Aim: The present study investigated the overlaid effect of ovariectomy on T2DM and the possible underlying mechanism. Materials: Forty female Wistar rats were divided into four groups (10 rats each): sham control, ovariectomized control, sham diabetic and diabetic ovariectomized groups. At the end of experiment, estimation of body weight gain percentage, food intake, fasting blood glucose concentration, and insulin tolerance test were done. Then, rats were euthanized and blood samples were taken for measurement of serum concentration of insulin, HOMA-IR, lipid parameters, tumor necrosis factor-a, interleukin 1 beta, interleukin 4, interleukin 10, malondialdehyde and total thiol. Also, histopathological and immunohistochemical examination of the pancreas were done. Results: The present study revealed that ovariectomy aggravated the diabetic induced glucose metabolic disturbance as implied by impaired insulin tolerance test, increased insulin resistance alongside lipid dyshomeostasis. These metabolic disturbances might claim to exacerbation of oxidative stress and inflammatory response along with apparent histopathological and immunohistochemical changes on the pancreas. Conclusion: We concluded that metabolic disturbances induced by diabetes might be getting worse after ovariectomy via intensification of oxidative stress and inflammatory state.
Citation: Abd El-Fatta et al., Effect of Tenofovir Administration and Withdrawal on the Kidney Tissues of Adult Male Albino Rats and the Protective Role of Vitamin E. SVU-IJVS 2019, 2 (2): 71-80.
AbstractTenofovir disoproxil fumarate is one of antiretroviral drugs used to treat chronic hepatitis B and HIV. In recent studies many Tenofovir problems were recorded; acute kidney injury (AKI), chronic kidney diseases (CKD) and Fanconi syndrome. Vitamin E is a powerful antioxidant that reduce free radical damage. Vitamin E supplementation can protect kidney tissues and enhances their regeneration if it is added to Tenofovir treatment. The aim of the present study is to describe the changes which occur in the kidney tissues of the adult male albino rats after Tenofovir administration and determine whether these changes are reversible or not after drug withdrawal, Also, whether vitamin E has a protective role or not. A total number of 40 adult male albino rats weighting 200-250 grams were used. Rats were divided into four groups, 10 rats in each group. The first group I (control group), The second group II which was treated with Tenofovir 10 mg/kg/day for two months, the third group III which was treated with Tenofovir10 mg/kg/day for two months, vitamin E also was given 100mg/kg/day for two months. The fourth group IV which was treated with Tenofovir 10 mg/kg/day for two months then withdrawal of Tenofovir for one month. There was marked degeneration of renal glomeruli and tubules in group II which was highly statistically significant than control group (P< 0.001), while in group III there was marked improvement of renal glomeruli and tubules which was highly statistically significant than group II (P<0.001). while in group IV these changes were moderately significant when compared with group II (P<0.01). Therefore, Vitamin E has a protective role if it is added to Tenofovir treatment, also vitamin E enhances regeneration of renal tissues after Tenofovir withdrawal.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.