Bengkirai is one of kind of woods usually used for furnitures and produces lots of woodcuts. This woodcut become the raw material for liquid smoke. The efficacy of liquid smoke produced from bengkirai wood against Phytophthora citrophthora was evaluated. The aim of this research was to evaluate antimicrobial properties of liquid smoke from bengkirai wood against P. citrophthora. Three kinds of liquid smoke were used in three temperatures i.e., 350, 400 and 450ºC. Efficacy of liquid smoke from bengkirai wood for antimicrobial used PDA medium. Simple linear regression was used to measured the effect of liquid smoke concentration to inhibition of P. citrophthora growth. The relationship between the concentration of liquid smoke (X) and inhibition of fungal growth, P. citrophthora (Y) at 350, 400, and 450ºC were Y = 24.51 + 5.27X (r2 = 0.98), Y = 54.31 + 5.53X (r2 = 0,92), and Y = 51.32 + 16.87X (r2 = 0.80). The results showed that the concentration of liquid smoke was significantly different for inhibition of P. citrophthora growth. The higher the concentration the higher the inhibition of microbial growth. The highest inhibition was on liquid smoke of bengkirai wood with temperature pyrolysis of 450ºC and concentration of liquid smoke of 1% with average value of 100%. IntisariBengkirai merupakan bahan baku pembuatan furniture yang banyak menghasilkan limbah potongan kayu. Limbah potongan kayu ini dapat dijadikan sebagai sumber pembuatan asap cair. Penelitian tentang efikasi asap cair dari kayu bengkirai terhadap Phytophthora citrophthora telah dilakukan. Penelitian ini bertujuan untuk mengevaluasi sifat antimikrobia asap cair kayu bengkirai dalam menghambat pertumbuhan P. citrophthora secara in vitro. Tiga jenis asap cair kayu bengkirai hasil pirolisis pada suhu 350, 400, dan 450ºC. Medium PDA digunakan untuk efikasi asap cair kayu bengkirai terhadap pertumbuhan P. citrophthora. Analisis regresi linier sederhana digunakan untuk mengevaluasi pengaruh konsentrasi terhadap daya hambat pertumbuhan P. citrophthora. Konsentrasi asap cair kayu bengkirai yang digunakan adalah 0; 0,5; 1,0; 1,5 dan 2,0 %, v/v. Hasil penelitian menunjukkan bahwa hubungan antara konsentrasi asap cair (X) dan daya hambat pertumbuhan P. citrophthora (Y) pada suhu pirolisis asap cair 350, 400, dan 450ºC berturut-turut adalah Y = 24,51 + 5,27X (r2 = 0,98), Y = 54,31 + 5,53X (r2 = 0,92), dan Y = 51,32 + 16,87X (r2 = 0,80). Makin tinggi konsentrasi asap cair kayu bengkirai makin tinggi daya hambat terhadap pertumbuhan P. citrophthora. Daya hambat pertumbuhan P. citrophthora tertinggi (100 %) pada perlakuan asap cair kayu bengkirai yang diproduksi pada suhu pirolisis 450ºC dan konsentrasi 1 %.
Candidiasis is a fungal infection caused by the fungus Candida albicans. One of the plants that has potential as an antifungal activity is Acalypha hispida. The objectives of this research determined the phytochemical analysis and antifungal activity of A. hispida flower methanolic extract on the growth of C. albicans using the disc diffusion method. Identification of compounds using thin layer chromatography (TLC). This study consisted of six treatments, namely control using ketoconazole and concentrations of A. hispida methanol extract of 0, 0.4, 0.6, 0.8, and 1.0 g/mL. The results showed that all concentrations of A. hispida methanolic extract had antifungal properties against the growth of C. albicans. The concentration of 1.0 g/mL showed the best inhibition against C. albicans. The methanol extract of A. hispida flowers contains alkaloids, flavonoids, terpenoids and phenols.
Antifungal Activity of Wood Vinegar derived from Oil Palm Empty Bunches against Colletotrichum sp. (WA2) Colletotrichum sp. is a fungus that causes anthracnose in cayenne pepper (Capsicum frutescens L.). An alternative natural control for this fungus is using wood vinegar. The aim of this study was to evaluate antifungal properties of wood vinegar from oil palm empty bunches (Elaeis guineensis Jacq.) against Colletotrichum sp. The antifungal test was carried out using the solid dilution method by poisoning food in potato dextrose agar (PDA) media. Colletotrichum sp. used was isolated from red chili which had anthracnose symptoms. This experimental research method used a completely randomized design (CRD) with 9 treatments, namely, negative control (non-liquid smoke), positive control (Dithane M45 at 0.20%), liquid smoke concentration of 0.40; 0.42; 0.44; 0.46; 0.48; 0.50; and 0.52%. Anova test results showed wood vinegar from empty fruit bunches of E. guineensis at concentrations of 0.40; 0.42; 0.44; 0.46 and 0.48% differed significantly from negative control, positive control, and concentrations of 0.50 and 0.52%. The concentration of 0.42% was the minimum inhibitory concentration with an average value of 87.98% inhibition and a very strong activity level. In conclusion, the wood vinegar from E. guineensis can be used to control Colletotrichum sp fungus at an effective concentration of 0.42%. Colletotrichum sp. merupakan jamur penyebab penyakit antraknosa pada tanaman cabai rawit (Capsicum frutescens L.). Salah satu alternatif pengendalian secara alami terhadap jamur tersebut adalah dengan menggunakan asap cair. Penelitian ini bertujuan untuk mengevaluasi sifat antijamur asap cair dari tandan kosong Elaeis guineensis Jacq (kelapa sawit) terhadap Colletotrichum sp. Uji antijamur dilakukan dengan metode dilusi padat melalui cara poisoning food dalam media potato dextrose agar (PDA). Isolat jamur Colletotrichum sp. yang digunakan merupakan hasil isolasi dari tanaman cabe merah yang bergejala antraknosa. Metode penelitian ini mengunakan rancangan acak lengkap (RAL) dengan 9 perlakuan, yaitu kontrol negatif (tanpa asap cair), kontrol positif (Dithane M45 sebesar 0,20%), konsentrasi asap cair sebesar 0,40; 0,42; 0,44; 0,46; 0,48; 0,50; dan 0,52%. Hasil uji Anova menunjukkan asap cair dari tandan kosong E. guineensis pada konsentrasi 0,40; 0,42; 0,44; 0,46; dan 0,48% berbeda secara signifikan dengan kontrol negatif, kontrol positif dan konsentrasi 0,50 dan 0,52%. Konsentrasi 0,42% merupakan konsentrasi hambat minimum dengan nilai rata-rata penghambatan sebesar 87,98% dan tingkat aktivitas sangat kuat. Dengan demikian dapat disimpulkan bahwa asap cair dari tandan kosong E. guineensis dapat digunakan untuk mengendalikan jamur Colletotrichum sp. pada konsentrasi efektif 0,42%.
The present study aims to optimize the processing variables producing liquid smoke from mabang wood (Shorea pachyphylla) by using Response Surface Methodology (RSM). In this investigation, a design of experiment with different combinations of pyrolysis temperature and pyrolysis time on the liquid smoke yield from mabang wood was applied. The response of the optimal yield, temperature, and time of pyrolysis was predicted using a mathematical model. The optimal operating conditions for the process of yielding 31.31% liquid smoke were identified at the pyrolysis temperature of 440◦C and pyrolysis time of 124 minutes. The effect of pyrolysis temperature was more significant than the pyrolysis time (p<0.05). The liquid smoke samples were evaluated by a GC-MS. The main chemical compound of the liquid smoke were 1,2-ethanediol (19.26%), fluoromethane (6.69%), formic acid (4.96%), 2-propanone (4.17%), acetic acid (18.64%), acetol (4.80%), furfural (9.94%), 2,4-hexadecanoic acid (3.45%), and guaiacol (2.93%).
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