Background: Chia seed is an oil-seed of multiple biological activities. Doxorubicin is an effective chemotherapy for liver cancer. Resistance and adverse effects are doxorubicin limitations. Objective: This study aimed to investigate the effect of chia seeds oil (CSO) on the resistance of HepG2 cells to liposomal-doxorubicin (DOX). Methods: The objective were investigated through measuring cytotoxicity, doxorubicin-metabolizing enzyme Cytochrome P450 3A4 (CYP-3A4), multidrug resistance-associated protein (MRP1), and the expression of multiple tumor suppressor microRNAs. Results: The findings indicated that low concentration of CSO increased HepG2 cells sensitivity to DOX as concluded from its higher cytotoxicity. DOX induced mRNAs of CYP-3A4 and MRP1 and their protein levels. CSO inhibited both in DOX-treated cells. CSO induced tumor suppressor miRNAs. Doxorubicin inhibited miR-122 and let-7/b/e expression, while it led to overexpression of let-7a. CSO/DOX upregulated let-7/b/e, miR-34a, and miR-122 (which inhibits MRP1) and downregulated let-7a that may lead to increased apoptosis. Conclusion: CSO effectively re-sensitized HepG2 cells to liposomal-doxorubicin via inhibiting MRP1 and CYP-3A4 that may increase in vivo doxorubicin bioavailability and decrease its therapeutic dose to diminish its adverse effects.
A total of thirty laying hens divided into 3 equal groups was used. The 1st group served as control and was fed a basal ration. The 2nd and 3rd groups received L-Dopa and reserpine, respectively. The goal of the study was devoted to determine the effects of these additives on the rate of egg production, weights of eggs and yolks as well as yolk proteins and their amino acid pattern.In the reserpine-treated hens the rate of egg production was increased whereas in the L-Dopa-treated group, the weights of eggs and yolks, food intake as well as the amino acids aspartate, glutamate, glycine, alanine and methionine were significantly increased. The levels of tyrosine and histidine were decreased in both treatments while methionine was decreased by reserpine only. The addition of dopamine antagonist to a ration containing lysine and leucine in concentrations of 0.55% and 0.9% did not influence their levels in eggs.The increment of methionine, aspartate, glutamate and glycine by the dopamine agonist and their decrease by antagonist revealed that these amino acids are excitatory neurotransmitters in brain.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.