SOR (Reactive Oxygen Species) is a collection of free radical compound or compounds that easily become free radicals and can damage the cell membrane is marked by elevated levels of MDA (11) This is called oxidative stress, which can be prevented and reduced by adequate intake of antioxidants into the body optimally. Oxidative stress effects can be prevented by antioxidant vitamins, especially vitamin E. The results of research show that the average value of mouse liver MDA levels of the control group (A) 1.78 nmol/mL. The treatment group with repeated delivery of cooking oil (B) have increased the average value of mouse liver MDA levels (2.97 nmol/mL, 3.18 nmol/mL, 3.49 nmol/mL, 3.83 nmol/mL). In the treatment group with doses of vitamin E 52 IU/Kg/Hr and repeat cooking oil (C) have decreased the average value of mouse liver MDA levels (2.03 nmol/mL, 2.10 nmol/mL, 2.24 nmol/mL, 2.70 nmol/mL), compared with the provision of cooking oil over it. We can conclude that there are differences in average MDA levels of the liver of mice a significant between group A, group B1, C1 group (p = 0.003). Mice liver MDA content significantly between group A, group B2, C2 group (p = 0.000), between group A, group B3, C3 group (0.001), between group A, group B4, C4 group (0.000) where p <0.05. Keywords: Recurring Cooking Oil, Vitamin E, Malondialdehid (MDA) PENDAHULUANPenggunaan minyak goreng tidak lepas dari kehidupan masyarakat. Masyarakat biasanya banyak mengkonsumsi minyak goreng yang berasal dari kelapa sawit. Minyak goreng kelapa sawit dapat berupa minyak goreng kelapa sawit yang bermerek dan dapat berupa tidak bermerek atau curah. Minyak goreng kelapa sawit yang tidak bermerek paling banyak dikonsumsi oleh masyarakat, terutama masyarakat menengah ke bawah. Minyak goreng kelapa sawit yang bermerek harganya relatif lebih mahal dibandingkan dengan minyak goreng kelapa sawit yang tidak bermerek.Suatu penelitian menunjukkan bahwa minyak goreng kelapa sawit yang bermerek dan tidak bermerek, dengan peningkatan suhu dan lama waktu pemanasan akan menyebabkan peningkatan kejenuhan asam lemak minyak goreng tersebut [1] . Pemanasan minyak goreng yang berulang akan rentan terhadap kerusakan oksidasi. Reaksi tersebut akan mengakibatkan perubahan/kerusakan baik secara fisika atau kimia. Perubahan ini dapat berupa kenaikan dan penurunan kualitas minyak goreng [2] . Pemanasan minyak goreng yang berulang sering dijumpai pada penjualan gorengan dipinggir jalan, dan ibu-ibu rumah tangga. Penggunaan minyak berulang bila dikonsumsi untuk jangka waktu yang lama, akan dapat membahayakan kesehatan. Penelitian Rustika menyatakan bahwa minyak goreng yang dipakai untuk memasak sebaiknya maksimal 4 kali, agar gorengan tidak banyak mengandung asam lemak jenuh yang dapat membahayakan kesehatan [3] . Minyak yang mengalami proses panas kedingin berulang sampai 4 kali, akan menurunkan mutunya karena mengandung banyak asam lemak jenuh yang dapat menyumbat pembuluh darah [4] .
AbstrakAkumulasi logam berat dapat meningkatkan senyawa oksigen reaktif dan menekan kadar antioksidan esensial dalam tubuh. Vitamin C merupakan antioksidan non enzimatis, senyawa alami yang bersifat antioksidan kuat dan pengikat radikal bebas. Penelitian ini bertujuan untuk mengetahui Efek Pemberian Vitamin C Terhadap Aktifitas Katalase Hati Tikus Galur Wistar Yang Terpapar Ion Pb, dilakukan di laboratorium Farmakologi Fakultas Farmasi dan Biokimia Fakultas Kedokteran Unand Penelitian dilakukan terhadap tikus galur wistar, berumur tiga bulan, berat badan + 200 gram, yang berjumlah 28 ekor. Didapatkan bahwa pemaparan ion Pb 0,05 mg/g BB/hari selama empat minggu dapat menurunkan aktifitas katalase hati tikus galur wistar. Penambahan vitamin C 0,05mg/g BB/hari dan 0,075 mg/g BB/hari pada tikus yang terpapar ion Pb menunjukan peningkatan aktifitas katalase hati tikus galur wistar.Berdasarkan uji T berpasangan terdapat perbedaan yang signifikan antara aktifitas katalase hati tikus galur wistar yang tidak terpapar ion Pb dengan terpapar ion Pb (p = 0,005 atau p < 0,05). Pemberian vitamin C pada tikus galur wistar yang terpapar ion Pb secara statistik dengan uji one way Anova tidak terdapat pengaruh yang signifikansi (p = 0,143 atau p > 0,05). Namun pada post hocx test terdapat pengaruh pemberian vitamin C 0,075 mg/g BB/hari pada tikus yang terpapar ion Pb, dengan p = 0,053 atau p = 0,05. Kata kunci: Aktifitas Katalase, Vitamin C, Ion Pb AbstractThe presence of heavy metals such as Pb can produce a free radical, it will also be able to decrease the availability of the body's antioxidants. Vitamin C is a non-enzymatic antioxidants, natural compounds that are strong antioxidants and free radical binding. This study to determine the Effect of Vitamin C to catalase activity of rat-wistar strain-liver. The purpose of which Exposed Pb ions, carried out in the laboratory of Pharmacology of the Faculty of Pharmacy and Biochemistry Faculty of Medicine Unand. Research conducted on wistar strain rats, three months old, weight + 200 grams, which totaling 28 tails. The result was exposure of Pb ions 0.05 mg / g BW / day for four weeks decreases catalase activity rat's-wistar-strain liver. The addition of vitamin C 0,05mg / g BW / day and 0.075 mg / g bw / day in exposed rats to Pb ions showed increased levels of catalase activity rat's-wistar-strain liver. The result of the paired t test there was difference significantly between liver catalase activity rat's-wistar-strain liver were not exposed to Pb ions (p = 0.005 or p < 0.05). Administration of vitamin C were exposed to Pb ion statistically by one-way ANOVA test there was no significant effect ( p = 0.143 or p > 0.05 ) . But in the post hocx test the effect of vitamin C contained 0.075 mg / g bw / day in rats exposed to Pb ions, with p = 0.053 or p = 0.05
Ultraviolet (UV) in addition to have a positive impact on health, can also endanger your health. UV negative impact is felt by the people who are exposed to UV light for long periods, such as fishermen and farmers. UV radiation levels are causing skin redness (erythema), whereas high levels can cause bleeding in the skin. This study aims to determine the effect. These are experimental studies using rats as an animal, which conducted the Biochemistry Laboratory of the Faculty of Medicine University of Newcastle from September -December 2008. Population is 20 strains of Wistar rats, age ± 2 months and weight 200-250 grams. Samples were taken at random (simple random sampling) of the population and divided into two groups: control group (group UV irradiated 6 hours/day for 3 weeks without vitamin E) and the treatment group (group UV irradiated 6 hours/day for 3 weeks with doses of vitamin E 0:45 IU/Kg BB/kali/hari). The results showed in the control group declines erytrosit and catalase enzyme activity due to exposure to UV. Declining sum was much less after vitamin E. The effect vitamin E can inhibit the decrease in the number erytrosit and catalase enzyme activities of rats exposed to ultraviolet light of vitamin E on the number of erytrosit and catalase enzyme activities of rats by exposure to ultraviolet light.
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