Donnellan, J. Edward, Jr.
(U.S. Army Natick Laboratories, Natick, Mass.),
Ella H. Nags, and Hillel S. Levinson
. Chemically defined, synthetic media for sporulation and for germination and growth of
Bacillus subtilis
. J. Bacteriol.
87:
332–336. 1964.—From 90 to 130 mg (dry weight) of spores (about 1% dark forms) were obtained (per liter) from a chemically defined, synthetic medium, with a two-phase (polyethylene glycol-potassium phosphate) harvest procedure. Optimal sporulation occurred when glucose and glutamic acid were at a concentration of 10 m
m
in the medium. Ca
++
and Mn
++
were required for sporulation. Heat resistance, dipicolinic acid content, and properties of germination and postgerminative development of spores grown in different concentrations of Ca
++
were investigated. Heat shock did not increase germination of spores derived from the synthetic medium. A synthetic medium, in which spore germination, emergence, and first cell division approached synchrony, was devised.
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