The growth of representative strains of lactobacilli in a laboratory scale distillery fermentation has been examined. Some strains were particularly deliterious to the fermentation and wore responsible for reducing the final pH, increasing the final gravity and reducing the alcohol concentration in fermented wash. The effects of varying degrees of infection by one deliterious strain and one innocuous strain were studied in more detail. Spirit yield depended on strain and inoculum size. In all cases the concentration of lactic acid was increased and the balance of other organic acids was disturbed by the presence of the bacteria.
Lyophilization, desiccation on pumice stone and storage in dilute glycerol at‐20d̀C were compared for the preservation of Lactobacillus cultures. After storage for two years, 73% of the lyophilized cultures were viable. Desiccation on pumice stone gave erratic results and is not recommended. Storage in 20% (v/v) glycerol at‐20d̀C is simple and convenient and after 24 months, 75% of the cultures were viable. It is a useful method of maintaining routinely accessed cultures.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.