SUMMARYWe describe in vitro conditions for packaging of exogenous DNA of Salmonella phage P22 which has terminally redundant, circularly permuted DNA. The method is a modification of the Kaiser-Masuda procedure. The most important aspect is to prepare all components (proheads, enzymes and concatemeric DNA) in end-cells. The influence of several factors such as DNA-and Mg ~ + concentration and kinetics has been investigated.
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