Cheese is a food that is highly susceptible to contamination by pathogenic and spoilage microorganisms, which can result in a decrease in its shelf life and cause serious risks to the consumers’ health. Consumers always require healthy food, free of synthetic preservatives, inducing a search for natural alternatives to ensure safety of the products. Essential oils and plant extracts emerge as an alternative for aiding cheese preservation. Some substances have demonstrated good effects against most pathogens and cheese spoilage microorganisms. However, intrinsic and extrinsic factors may influence the actions of these compounds when incorporated into cheese, besides affecting the product characteristics. This review aims at discussing the antimicrobial efficiency of plant extracts and essential oils as well as the impact of their incorporation on lactic bacteria and the sensory characteristics of products.

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Beer is the most consumed beverage in the world, especially in countries such as USA, China and Brazil.It is an alcoholic beverage made from malted cereals, and the barley malt is the main ingredient, added with water, hops and yeast. High-pressure processing is a non-traditional method to preserve food and beverages. This technology has become more interesting compared to heat pasteurization, due to the minimal changes it brings to the original nutritional and sensory characteristics of the product, and it comprises two processes: high hydrostatic pressure, which is the most industrially used process, and high-pressure homogenization. The use of high pressure almost does not affect the molecules that are responsible for the aroma and taste, pigments and vitamins compared to the conventional thermal processes. Thus, the products processed by high-pressure processing have similar characteristics compared to fresh products, including beer. The aim of this paper was to review what has been investigated about beer processing using this technology regarding the effects on physicochemical, microbiology and sensory characteristics and related issues. It is organized by processing steps, since high pressure can be applied to malting, mashing, boiling, filtration and pasteurization. Therefore, the beer processed with high-pressure processing may have an extended shelf-life because this process can inactivate beer spoilage microorganisms and result in a superior sensory quality related to freshness and preservation of flavors as it does to juices that are already commercialized. However, beyond this application, high-pressure processing can modify protein structures, such as enzymes that are present in the malt, like α- and β-amylases. This process can activate enzymes to promote, for example, saccharification, or instead inactivate at the end of mashing, depending on the pressure the product is submitted, besides being capable of isomerizing hops to raise beer bitterness. As a consequence, the process may reduce steam demand and residue generation. Therefore, the use of high-pressure processing can potentially replace or be combined with heat processes usually applied to beer, thus bringing benefits to the sensory quality of the product and to the environment.

A vigilância em saúde possui como objetivo a observação e análise constantes da situação de saúde da população. As doenças transmitidas por alimentos e seus surtos são um dos maiores desafios para a Saúde Pública. Estes surtos devem ser notificados, segundo o Sistema Nacional de Vigilância Epidemiológica vigente, sendo que, paralelamente à investigação, devem ser tomadas medidas de prevenção e controle. No Brasil esse perfil epidemiológico ainda é pouco conhecido, presumindo-se alta morbidade sem conhecer sua real magnitude. Buscando diminuir os casos destes surtos, a Secretaria do Estado de São Paulo aprovou em abril de 2013 a Portaria CVS no 5. Neste regulamento foram acrescidas medidas de prevenção e controle importantes para assegurar a diminuição do risco de contaminação do alimento. Este trabalho teve como objetivo discutir os avanços da legislação em relação à segurança dos alimentos, analisando as diretrizes da nova Portaria CVS no 5/2013. Constatou-se que é uma legislação mais rígida e detalhada que as revogadas CVS no 6/1999 e CVS no 18/2008 e até mesmo que a legislação federal em vigor, RDC no 216/2004, principalmente no que tange a saúde e segurança do manipulador, processamento e armazenamento dos alimentos.

ResumoO presente estudo teve como objetivo caracterizar a termorresistência dos fungos mais termorresistentes, isolados de néctares de maracujá e abacaxi comerciais pasteurizados, bem como verificar a produção de patulina por esses fungos, quando inoculados em tais néctares. Os fungos mais termorresistentes isolados foram o Byssochlamys fulva em néctar de maracujá e o Byssochlamys nivea em néctar de abacaxi. O B. nivea, em néctar de abacaxi, mostrou-se mais termorresistente, com base nos valores de D 98°C e Z, os quais resultaram em 27 min e 5,4 °C, respectivamente, em comparação com 13,6 min e 5,5 °C encontrados para B. fulva, em néctar de maracujá. A pasteurização comumente aplicada pela indústria não se mostrou suficiente para inativação dos fungos isolados. Não

This study was carried out to evaluate the antilisterial activity of carrot, and the impact of its incorporation on the carotenoid content in Minas Frescal cheese, combined or not with high hydrostatic pressure processing, as an alternative for the development of healthier fresh cheese free of synthetic preservatives. Cheeses were manufactured with milk added with carrot incorporation (0, 3, and 6%) and pressurized (0; 250 and 500 MPa/10 min). Total carotenoid content, α-carotene, β-carotene, lutein, consumer acceptance, and sensory characteristics were determined one day after the manufacture while Listeria innocua enumeration was evaluated up to 22 days of storage at 8 ℃. The results showed that although a decrease of over 7 log CFU g−1 in L. innocua counts was observed immediately after fresh cheese processing at 500 MPa/10 min, inactivation was not complete, as the growth of this nonpathogen surrogate during storage was observed. The addition of 6% carrot had a slight bacteriostatic effect, verified on the 15th day of storage, particularly in pressurized cheeses. On the other hand, high pressure treatment at 500 MPa/10 min increased carotenoids degradation in cheeses. Although pressurized cheeses were characterized as “rubbery,” high hydrostatic pressure had no significant effect on consumer acceptance.

RESUMO Azeitona de mesa é o produto vegetal fermentado de maior importância no mundo ocidental. Apesar de não

Brazil nut is a typical oilseed from the Amazon region, of great economic and cultural importance. Due to its high nutritional value, it is interesting to be used as a matrix for new products’ development. Thus, the present study aimed to develop and characterize a drink based on Brazil nuts fermented by Lactobacillus casei and added with inulin. After formulated, the drink was pasteurized, fermented, maturated, and stored under refrigeration for 28 days. Physical, chemical, microbiological, and sensory analyzes were performed before and after fermentation, at 7-day intervals. There was maintenance in the content of lipids and proteins and reduction in the concentrations of fibers, ash, total carbohydrates, and moisture. The fermented drink pH decreased significantly during storage, while the acidity and the soluble solids content increased from the 14th day. Regarding the color, there were significant changes in all parameters analyzed. In the fermented drink, the development of molds and yeasts, total and thermotolerant coliforms, and Salmonella were not verified, while the control drink deteriorated after 7 days. The probiotic L. casei had count ranging from 9.48 to 8.59 log CFU mL-1 during the 28 days. When exposed to in vitro gastrointestinal conditions, L. casei significantly reduced its concentration. However, it reached the enteric phase with counts greater than 6.00 log UFC mL-1. Sensorially, the drink reached intermediate scores (between 5.41 and 6.02). Therefore, the use of Brazil nuts proved to be viable for the development of a product free of animal-origin components, symbiotic, and of outstanding nutritional quality.