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Simulation and experimental measurements were used aiming at better understanding flow behavior in planar and three-dimensional microchannels used for separation or preconcentration. Two different microchannels configurations were designed, manufactured in acrylic and the surface was modified to adsorb volatile organic compounds (VOCs). Both channels configuration are 73 cm long, 100 µm wide and 40 µm deep. Simulation was carried out in COMSOL (FEMLAB) 3.2 ® and COSMOS flow 5.0 ® programs, tracer visualization and quartz crystal microbalance data were evaluated according to chromatographic concepts. Due to the higher axial velocity, planar microchannels are more adequate for preconcentration whereas three-dimensional ones show a good performance in the separation process.Keywords: Microchannels; Planar, Three-dimensional, Preconcentration. Resumo Foram usadas simulações computacionais e medidas experimentais com o objetivo de compreender o comportamento de fluidos em microcanais tridimensionais e planares que foram testados na função de separação ou pré-concentração. Duas configurações diferentes foram projetadas, construídas em acrílico e tiveram sua superfície modificada para adsorver compostos orgânicos voláteis (COVs

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