A B S T R A C TSalmonella species have been the major foodborne problems in food production systems, with Salmonella enterica serovars typhimurium (S. typhimurium) and enteritidis (S. enteritidis) being among the more common isolates. The oral administration of chicken egg yolk specific antibodies (IgYs) has been established as an efficient alternative for treatment and prevention of gastrointestinal pathogens including Salmonella. The present study was aimed to investigate the possible production of specific IgYs against Salmonella typhimurium and Salmonella enteritidis in quail egg yolks. Salmonella spp.-free female Japanese quails (Coturnix coturnix japonica) were intramuscularly immunized with formalin or heat-inactivated Salmonella immunogens (1.0 × 10 9 CFU/mL) emulsified with Freund adjuvants. Egg yolk IgYs were purified using ammonium sulfate precipitation method. Anti-Salmonella IgYs titer and specificity were determined using enzyme-linked immunosorbent assay (ELISA) and western blot analysis. Salmonella specific IgYs detected in the immunized quails were significantly higher than those of the control group, which confirmed the immunization procedure. Specific IgYs against S. typhimurium and S. enteritidis were identified in both groups immunized with heat or formalin-inactivated immunogens. However, formalin-inactivated immunogens induced relatively higher immune responses over the heat-inactivated ones. Quail anti-Salmonella IgYs showed a high specificity to their corresponding immunogens, with moderate crossreactivity to other members of Enterobacteriaceae family. Quail can be regarded as a valuable and inexpensive source for producing large-scale of specific antibodies that can be used for immunodiagnostic and immunotherapeutic purposes.

Chicken egg yolk antibodies (IgYs) are extensively used for immunotherapy and immunodiagnostic purposes. Oral immunotherapy with specific IgYs is established as an efficient alternative to traditional antibiotic therapy in human and animals. Storing immunized eggs in refrigerator for a period of time could provide an inexpensive and convenient source of large volumes of specific antibodies. This study investigated the storage stability of anti- Salmonella Typhimurium IgYs in immunized quail egg yolks at 4°C over a period of more than 6 months. Salmonella spp.-free female Japanese quails (Coturnix coturnix japonica) were intramuscularly immunized whit Salmonella Typhimurium whole bacterial suspension (1.0×109 CFU/ml) emulsified with Freund adjuvants. During a period of 10 days after final immunization, eggs from each group were collected, randomized and stored at 4°C over a period of 200 days. Egg yolk IgY titer and specificity were determined using ELISA technique. S. Typhimurium specific IgY antibodies were detected in immunized quails and were significantly higher than the control group which confirmed the immunization procedure. Eggs from immunized quails can be collected and stored in 4°C refrigerator over a period of 2 months without any concern about the antibody degradation. After 80 days of storage at 4°C, although lower antibody titer was obtained in comparison to the first of study, anti-S. Typhimurium IgY level remained stable up to the 6 months without more significant declining. This trend will provide economical sources of polyclonal antibodies through reducing the number of immunized animals, management expenses and housing costs.