Introduction: Alzheimer’s disease (AD), which is a progressive neurodegenerative disorder, causes structural and functional brain disruption. MS4A6A, TREM2, and CD33 gene polymorphisms loci have been found to be associated with the pathobiology of late-onset AD (LOAD). In the present study, we tested the hypothesis of association of LOAD with rs983392, rs75932628, and rs3865444 polymorphisms in MS4A6A, TREM2, CD33 genes, respectively.
Methods: In the present study, 113 LOAD patients and 100 healthy unrelated age- and gender-matched controls were selected. DNA was extracted from blood samples by the salting-out method and the genotyping was performed by RFLP-PCR. Electrophoresis was carried out on agarose gel. Sequencing was thereafter utilized for the confirmation of the results.
Results: Only CD33 rs3865444 polymorphism revealed a significant difference in the genotypic frequencies of GG (P = 0.001) and GT (P = 0.001), and allelic frequencies of G (P = 0.033) and T (P = 0.03) between LOAD patients and controls.
Conclusion: The evidence from the present study suggests that T allele of CD33 rs3865444 polymorphism is associated with LOAD in the studied Iranian population.
Mixer torque rheometry was used to investigate the rheological behavior of wet granulations with different concentrations of drug, binder, and water. An experimental design was employed to systematically study the effects of the three formulation variables on the torque profiles of the wet masses over time. Under comparable conditions, increasing binder and water concentrations tended to produce higher wet mass consistencies. Friability of the dried granules was measured as an indication of the strength of the granules. A reduced quadratic model in terms of each of the three variables was found to satisfactorily predict granule friability. Granule friability decreased with increases in the binder level and increased slightly with increasing drug concentration. An inverse relationship was seen between granule friability and the amount of water added to the formulation, especially at lower drug concentrations. Mixer torque rheometry is a useful method for studying the properties of wet granulations when minimal amounts of drug are available for the development of a wet granulated formulation.
The original version of this article unfortunately contained a mistake in the Authorgroup section. Author Azra Delpak's given name was misspelled as "Azar".
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