Highlights The vaccine is composed of immunodominant regions of SARS-CoV-2 non-structural proteins. Also, the functional region of the spike protein is incorporated in the vaccine construct. The final vaccine construct contains multiple CD8+ and CD4+ overlapping epitopes Also, it contains multiple IFN-γ inducing, linear and conformational B cell epitopes. It forms significant interactions and stable complex with TLR-4/MD. The DNA vaccine is designed by reverse translation of the final vaccine construct.

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