Strictly aerobic acetic acid bacteria (AAB) have a long history of use in fermentation processes, and the conversion of ethanol to acetic acid for the production of vinegar is the most wellknown application. At the industrial scale, vinegar is mainly produced by submerged fermentation, which refers to an aerobic process in which the ethanol in beverages such as spirits, wine or cider is oxidized to acetic acid by AAB. Submerged fermentation requires robust AAB strains that are able to oxidize ethanol under selective conditions to produce high-titer acetic acid. Currently submerged fermentation is conducted by unselected AAB cultures, which are derived from previous acetification stocks and maintained by repeated cultivation cycles. In this work, submerged fermentation for vinegar production is discussed with regard to advances in process optimization and parameters (oxygen availability, acetic acid content and temperature) that influence AAB activity. Furthermore, the potential impact arising from the use of selected AAB is described. Overcoming the acetification constraints is a main goal in order to facilitate innovation in submerged fermentation and to create new industry-challenging perspectives.
Some polyphenolic catabolites, generated in vivo in the colon, were able in vitro to counteract two key features of diabetic complications, i.e. protein glycation and neurodegeneration. These observations could lead to a better control of these events, which are usually correlated with hyperglycemia.
The ability of high molecular weight melanoidins extracted from coffee, barley coffee, and dark beer to inhibit lipid peroxidation during simulated gastric digestion of turkey meat has been investigated. Results showed that melanoidins decrease the synthesis of lipid hydroperoxides and secondary lipoxidation products. Coffee melanoidins at 3 mg/mL reversed the reaction and broke down hydroperoxides to concentrations lower than the initial value. Barley coffee and dark beer melanoidins were less effective, and even at 12 mg/mL did not reverse the reaction. The proposed mechanism of action involved Fe(2+) chelating capacity, heme-binding ability, and radical-scavenging activity. Melanoidins were characterized for their content in total proteins, carbohydrates, and phenolics, and the relationship between the chemical composition and the antioxidant activity of dietary melanoidins was investigated. Coffee melanoidins, which contain more phenolics and proteins with respect to the other melanoidins, showed greater antioxidant activity with respect to the other melanoidins tested.
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