Background/Aims: The antigen phenotype of human osteoblast-like cells suggests that they are related to other cellular populations and may also have immunologic functions in common. Methods: Flow cytometry and transmission electron microscopy were used to show the phagocytotic activity of osteoblast-like cells in culture. The allogeneic stimulation of T cells by human osteoblast-like cells was determined by the measurement of T cell proliferation. Results: We demonstrated in vitro that human osteoblast-like cells isolated from normal bone specimens obtained during mandibular osteotomy can phagocytose particles of different nature and size and can stimulate allogeneic T cells. Phagocytosis of microorganisms (E.coli, Klebsiella or C. albicans) was observed, although at a very low rate of activity in comparison with the phagocytosis of latex particles. Conclusion: Our results suggest that human osteoblast-like cells may perform immunologic functions and act as antigen presentation cells.p>
TGFbeta1 treatment significantly reduced the expression of CD54 and CD86. IL-1beta treatment significantly enhanced the expression of CD54, CD86 and HLA-DR. LPS and IFNgamma treatments produced a major increase in CD54, CD80, CD86 and HLA-DR expression. Expression of these antigen-presenting molecules was not significantly modified by FGFb, PDGF-BB or IL-2 treatment.
The antigenic phenotype of cultured human osteoblast-like cells, their ability to phagocytose particles of different nature and size, and their capacity to stimulate allogeneic T cells suggest that they are related to other cell populations with which they may also have immunological functions in common. The objective of this study was to investigate the intracytoplasmatic presence of cytokines and their modulation by different biomolecules. Immunocytochemistry and flow cytometry were used to study the expression of IL-4, IL-12, IL-15, IL-18, and IFNgamma cytokines. To investigate whether FGF, TGF, PDGF, IL-1, and IFNgamma modulate expression of these cytokines in cultured human osteoblast-like cells we used flow cytometry. IL-4, IL-12, IL-15, IL-18, and IFNgamma cytokines were expressed by all the cultured human osteoblast-like cells studied. Treatment with FGF and TGFbeta1 reduced the percentage expression and fluorescence intensity of the cytokines. PDGF treatment enhanced their fluorescence intensity but did not modify their expression. IL-1 treatment produced a small reduction in expression and fluorescence intensity of IL-12 and IL-15, but did not produce major changes in the expression of IL-4, IL-18, or IFNgamma. IFNgamma markedly increased the fluorescence intensity of the cytokines. The results indicate that human osteoblast-like cells may perform immunological functions (e.g., synthesizing cytokines with immune regulator function) that can be modulated by different biomolecules related to bone tissue and/or immune response.
La comprensión lectora es la capacidad de un individuo de captar lo más objetivamente posible lo que un autor ha querido transmitir a través de un texto escrito. Por lo tanto, la comprensión lectora (Reading Comprehension) es un concepto abarcado por otro más amplio que es la competencia lectora (Reading Literacy). La competencia lectora es la habilidad de un ser humano de usar su comprensión lectora de forma útil en la sociedad que le rodea. De esta forma, la comprensión lectora es el hecho abstracto dependiente de la capacitación individual de cada persona y la competencia lectora la materialización concreta llevada a cabo en dependencia de la relación del individuo con la sociedad.Así, la comprensión lectora está ligada más al individuo que al entorno, a sus capacidades intelectualeso emocionales, o su perfil psicológico, mientras que la competencia lectora añade más peso a una variable pragmática, la socialización, la inteligencia social o la inteligencia ejecutiva (Marina, 2012).
La comprensión lectora es la capacidad de un individuo de captar lo más objetivamente posible lo que un autor ha querido transmitir a través de un texto escrito. Por lo tanto, la comprensión lectora (Reading Comprehension) es un concepto abarcado por otro más amplio que es la competencia lectora (Reading Literacy). La competencia lectora es la habilidad de un ser humano de usar su comprensión lectora de forma útil en la sociedad que le rodea. De esta forma, la comprensión lectora es el hecho abstracto dependiente de la capacitación individual de cada persona y la competencia lectora la materialización concreta llevada a cabo en dependencia de la relación del individuo con la sociedad.Así, la comprensión lectora está ligada más al individuo que al entorno, a sus capacidades intelectualeso emocionales, o su perfil psicológico, mientras que la competencia lectora añade más peso a una variable pragmática, la socialización, la inteligencia social o la inteligencia ejecutiva (Marina, 2012).
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