An analysis and optimization of a test to quantitatively assess the resistance of disinfectants to neutralization by organic soil is presented. The recommended method uses sterile, dry baker’s yeast as a standard organic soil, and 24-hr cultures of Staphylococcus aureus and Pseudomonas aeruginosa as test organisms. The test determines the maximum per cent (w/v) of organic soil which a disinfectant can tolerate and remain able to kill about 106 test organisms/ml in 10 min at 25°C. This per cent is defined as the organic soil neutralization number. The procedure offers several advantages over other organic soil capacity tests.
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