Nile-Tilapia (Oreochromis niloticus) aquaculture represents one of the most important cultivation species in Egypt. However, Tilapia fish farming is challenged by some problems. Of those, the presence of bacterial pathogens resulting in high fish mortalities and huge economic losses. Thus, the current investigation aimed to isolate, identify, and characterize the pathogenic bacteria from Nile tilapia fish farm in El-Abassa village, Egypt and to investigate their antibiotic susceptibility as a primary step for controlling diseases. 182 bacterial isolates were obtained from one hundred Tilapia fish samples. The microbiological and biochemical analysis of the examined fish indicated the presence of only 5 bacterial genera. Three of them are Gram-negative bacteria (representing 86.26% of total isolates) including Aeromonas spp. (46.70 %), Pseudomonas spp. (23.08 %), and Vibrio spp. (16.48 %). While two genera are Gram-positive bacteria (representing 13.74% of the total isolates) including Streptococcus spp. (8.79 %) and Staphylococcus spp. (4.95 %). This indicates that Gramnegative bacteria are the main cause of high fish mortalities in the studied area while Aeromonas hydrophila exhibited the highest prevalence in infected tilapia. Antibiogram test revealed high levels of resistance expressed by all isolates to ampicillin, amoxicillin, and erythromycin. On the other hand, norfloxacin was effective against all isolated bacteria followed by ciprofloxacin; therefore, norfloxacin should be 24recommended as a supplement in fish fed-diets to control the bacterial infection. Establishing effective control methods for pathogenic isolates would greatly enhance fish production.
Thirty mastitic milk samples were collected from she camels for the isolation and identification of bacteria causing mastitis and to determine their antibiogram against certain antibiotics. Bacteriological examination of mastitic milk samples revealed 18 single isolates (60%) and 12 mixed isolates (40%). Gentamicin was found to be the highest effective drug against the isolated bacteria than other used drugs. A total of 25 she camels (15 healthy and 10 mastitic) were divided into 5 equal groups receiving gentamicin alone and/or in combination with isoflupredone acetate. Blood and milk samples were taken from all she camels at 1 st , 7 th and 15 th days post treatment for haematological and biochemical analysis.
The present study aimed to investigate the prevalence of Campylobacter jejuniand its clinicopathological changes in broiler chickens in Sharkia province. About 50 diarrheic broilerchicks' cloacal swabs were collected for bacteriological examination. Out of 50 examined swabs; 12 (24%) were positive for Campylobacter [4; C. coli 4 and 8; C. jejuni]. Isolated Campylobacterwassensitive to neomycin and gentamycin. About 45 healthy one-day-old broilerchickens received 5 mg pefloxacin/kg Bw for 5 days to exclude bacterial infections. At 14 th day broilers were divided into 3 groups (15/ each). First group; healthy broilers non-treated (control),broilers in 2 nd and 3 rd groups were infected with C. jejuni. 2 nd group were infected and nontreated, while 3 rd group infected, and treated with 15 mg neomycin/kg Bw. in drinking water for 5 days. At 1 st ,7 th and14 th day post treatment cloacal swabs were collected for re-isolation C. jejuni besideblood samples were collected for hematobiochemical study.Infectedbroilers showed offfood, depression, ruffled feather, diarrhea and mortality rate 40% beside significant decrease in body performance, total protein albumin and non-significant decrease in globulin coupled with non-significant elevation in RBCs, HB, PCV%, significant elevation in WBCs, AST, ALT, ALP, urea and creatinine.Treatment infected broilers byneomycin lead to disappear clinical sing, reduced mortality rate and improved hematobiochemical parameters. It could be concluded thatCampylobacter infection induces reversible adverse effect on body performance andhematobiochemical parameters. Neomycin is highly curative against campylobacters
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