Ochratoxin A (OTA) formation prevention is not usually available so counteracting strategies are urgent. This study investigated the toxic effects of OTA on Nile tilapia with trials to neutralize these effects by using some feed additives. Supplements used as one percent (Garlen Extra4, Nigella sativa, Garlen Extra4 plus Nigella sativa and a commercial mycotoxins binder, Fero Bind Pro) were added to 500 ppb/kg OTA-contaminated diet. Fish were fed at 3% body weight per day for 10 weeks. The clinical signs recorded in OTA – intoxicated positive control group were sluggish swimming and off food before death with reduction in survivability (53%) and growth performance. Several post-mortem lesions were in liver, kidneys and spleen. Serum levels of ALT, AST, creatinine and urea were significantly increased with reduction in total protein, albumin and globulin in ochratoxicated fish group compared to the negative control group. Concerning the pathological changes that have been noticed in ochratoxin treated fish were almost completely alleviated in examined tissues of fish that were fed on diet with Garlen Extra4 plus Nigella sativa or Nigella sativa and partially in fish that were fed on diet with Garlen Extra4 or Fero Bind Pro, respectively. Best detoxifying results were obtained by using 30 g/kg Nigella sativa plus 0.1 g/kg Garlen Extra4 followed by Nigella sativa, Garlen Extra4 then Fero Bind Pro as a commercial Mycotoxins binder. It could be concluded that inclusions of 30 g/kg Nigella sativa plus 0.1 g/kg Garlen Extra4 in Oreochromis niloticus (O. niloticus) diets could partially reduce OTA toxic effects.
Pedicoccus acidi lacti, S. enteritidis, broiler chicks, growth performance, blood biochemistry, hematology, NDV.The present study was conducted to investigate the effect of Pedicoccus acidi lacti supplementation to the ration of broiler chicks on growth performance, survival rate, immunity, hematological studied, serum biochemistry, response to NDV and its protective effect against artificial infection with S. enteritidis . One hundred and twenty day-old broiler chicks were allotted into four equal groups: group one fed on non-supplemented ration and not infected, group two fed on ration contain Pedicoccus acidi lacti 100 mg/kg ration and not infected, group three fed on non-supplemented ration and infected and group four fed on ration contain Pedicoccus acidi lacti 100 mg/kg ration and infected with salmonella. The results indicated that Pedicoccus acidi lacti supplementation improved the body weight and FCR in comparison to control group during the first two weeks (pre-infection), After infection the results indicated that the Pedicoccus acidi lacti supplementation protect chickens against bad effect of salmonella infection where both parameter (FCR and Body weights) still superior till 5th week. Also the addition of probiotics improved lipid profile and CBC. In the same time. It was evident that addition of Pedicoccus acidi lacti improved immune response to ND.
This experiment was conducted to investigate the effects of phytase, on growth performance, carcass characteristics and some biochemical parameters of Japanese quail fed on low phosphorus diet. Japanese quail were divided into 5 equal groups, each group subdivided into 2 subgroup. One of them contain 16 males while the other contain 16 females. Japanese quail supplemented with five diets in both Males and females. First group (Positive control) fed on diet contain recommended level of available phosphorus N.R.C. (1994), second group (negative control) fed on diet contain 2/3 recommended level of available phosphorus while the other three groups fed on negative control diet supplemented with (300,600 and 900 phytase enzyme unit FTU/Kg), respectively. growth performance results showed that body weight gain of negative control group significantly lowered relative to other groups while Feed conversion ratio of the adequate phosphorus groups or of the low phosphorus groups supplemented with phytase significantly better than the negative control groups in both males and females. The data interested in carcass characteristics showed that dressing percentage. breast and thigh muscles were improved in all dietary treatments in both males and females compared with negative control and significantly increased in groups fed on diet supplemented with (600 &900 phytase enzyme unit FTU/Kg when compared with the positive control in case of males However leg muscle increased significantly in case of female (900 phytase enzyme unit FTU/Kg) when compared with the positive control. The present data revealed that serum globulin significantly increased in male group fed low phosphorus diet supplemented with 900 phytase relative to other male groups, the serum phosphorus of female group fed low phosphorus diet supplemented with 900 phytase showed significantly higher relative to other female groups.
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