A mold which was isolated from soil produced a large amount of extracellular dextranase in the medium containing dextran. From the comparative taxonomic experiments, the mold was identified as a strain of Aspergillus ustus.When the dextrans produced by various strains of Leuconostoc mesenteroides were used as C-source, they had influence evidently on the productivity of dextranase.Especially, using the dextran produced by Leuconostoc mesenteroides D 12 was isolated from a waste water in our sugar refinery, 330 units of the dextranase per ml of culture broth was produced by the shaking culture at 30°C for 4 days.While the other dextrans gave a lower productivity than the above result, it was appeared that the dextranase productivity was not related to the structure of dextran. When some carbohydrates were separately added to the medium with dextran, the productivity of dextranase was inhibited by fructose, lactose or some sugar alcohols.
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