We examined Notch signaling molecules, Notch1 and Jagged1, in serial large cases of typical solid/multicystic ameloblastoma. In general, Notch positive staining products were frequently detected in the cytoplasms of the cells. In the same cells, Jagged positive staining were also frequently observed, while only occasionally positive in peripheral cells, especially in cuboidal cells. The results showed that these morphogenesis regulation factors are closely related to cytological differentiation in neoplastic cells of ameloblastoma. The Notch and Jagged positive-cell ratios were frequently positive, and the ratios were nearly the same between the varied histopathological, cytological patterns. However, the less-differentiated cells were fewer in number than that of well-differentiated cells.
A case of ameloblastoma in a 67-year-old male Malay patient was reported with examination of Notch signaling. After histopathological observations and diagnosis, the distribution of transcription factors and mRNA of Notch1 and Jagged1 were examined by immunohistochemical (IHC) and in situ hybridization (ISH) techniques. Histopathologically, the tumor consisted predominantly of proliferating follicular nests of ameloblastoma epithelium randomly disposed in the fibrous stromal tissue. Some of these odontogenic epithelial nests showed features of central cystic degeneration, squamous metaplasia, and keratinizing pearl formation. By IHC, Notch1 and Jagged1 positive products were observed in most of these proliferating nests of ameloblastomas. Both of these gene expressions were detected by ISH in the cytoplasms of these IHC positive cells. These preliminary findings suggest that these genes may play a role in cytological differentiation or acquisition of tissue-specific characteristics in neoplastic cells of ameloblastomas.
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