Production of reactive oxygen species (ROS) yields serious damage oxidation of proteins, lipids and genomic structures. Studies have shown that production of ROS increases during intensive exercise training. This study aimed to investigate the effect of high intensity interval training on 8-oxoguanine DNA glycosylase (OGG1) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in the brain and liver of rats. 16 adult Albino Wistar rats were randomly divided into sedentary control and high intensity interval training groups. Animals ran on treadmill for 6 weeks, 6 days per week, at 95 to 100 percent of maximal oxygen consumption. Using commercial kits, the content of OGG1 and 8-OHdG were measured using sandwich ELISA assay. Data analyzed using Student's Ttest at P≤0.05 level. High intensity interval training resulted in significant increases in contents of OGG1 in brain (t14=7.22, P=0.001) and liver (t14=2.55, P=0.02) of rats. However, high intensity interval training had no significant influence on 8-OHdG levels in brain (t14=1.60, P=0.13) and liver (t14=1.28, P=0.22) of rats. Also, there were no significant differences between changes in the brain and liver contents of OGG1 (t14=0.97, P=0.34) and 8-OHdG (t14=0.42, P=0.68) of rats following high intensity interval training. Taken together, separation of the training sessions to various bouts of exercise with maximum effort, through increase in OGG1 contents, will lead to modify of 8-OHdG levels in brain and liver.
The purpose of this study was to investigate the effect of intensive endurance training on 8 -oxoguanine DNA glycosylase (OGG1) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in liver and brain of rats. 16 adult and male albino wistar rats were randomly divided into sedentary control and endurance exercise training groups. Animals ran on treadmill for 6 weeks, 6 days a week, at a speed of 10 m/min (85 percent of maximal oxygen consumption). The content of OGG1 and 8-OHdG were measured using sandwich ELISA assay. Data analyzed using Student's T-test at P≤0.05 level. Our results showed that intensive endurance training has no significant effect on 8-OHdG contents in liver (t14=1.09, p=0.29) and brain (t14=0.93, p=0.36) of rats. However, contents of OGG1 in liver (t14=5.84, p=0.001) and brain (t14=4.09, p=0.001) of rats significant increases following intensive endurance training. Finally, there were no significantly differences between changes in contents of p=0.66) and OGG1 (t14=1.72, p=0.10) in liver and brain of rats following endurance training. Intensive endurance training maintains 8-OHdG genomic damage in baseline level in liver and brain of rats by increasing contents of OGG1.
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