Phylogenetic analyses of 165 rRNA gene sequences showed that the Gramnegative aromatic-and chloroaromatic-degrading Pseudomonas sp. strain HV3 carrying the mega-plasmid pSKY4 belongs to the genus Sphingomonas. The 16SrRNA sequence is most related to Sphingomonas chlorophenolica strains ATCC 33790T (9805%) and SR3 (984%) and Sphingomonas sp. SS86 (98.4%). The G+C content was 64 mol%, and the DNA-DNA-hybridization-based relative homology of strain HV3 to the 5. chlorophenolica ATCC 33790T and 5. chlorophenolica RA2 was 596 YO and 35-9 %, respectively. The results showed that although strain HV3 is related to S. chlorophenolica it differs in certain characteristics. It is therefore proposed to reclassify Pseudomonas sp. strain HV3 as Sphingomonas sp. HV3.
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