Severe thunderstorms are a common occurrence in Australia, and have been documented since the first European settlement in 1788. These events are characterized by large damaging hail in excess of 2cm, convective wind gusts greater than 90kmh-1 and tornadoes, and contribute a quarter of all natural hazard related losses in the country. This impact has lead to a growing body of research and insight into these events. In this article, the state of knowledge regarding their incidence, distribution, and the resulting hail, tornado, convective wind risk and lightning will be reviewed. Applying this assessment of knowledge, the implications for forecasting, the warning process and how these events may respond to climate change and variability will also be discussed. Based on this review, several potential avenues for future research and exploration are suggested, and ongoing work in the field outlined. Most notably, the need for improved observational or proxy climatologies, forecasting guidelines for tornadoes and a greater understanding of how severe thunderstorms respond to climate variability are highlighted.

Staphylococcus aureus enhances neutrophil extracellular vesicle (EV) production. To investigate whether S. aureus viability influences EV biogenesis, EVs were isolated from human neutrophils incubated with viable bacteria (bEVs) or heat-killed bacteria (heat-killed EVs). Protein analysis, nanoparticle tracking and transmission electron microscopy showed comparable EV production between subsets, and both viable and nonviable bacteria were also detected in respective EV subsets. As anticipated, S. aureus, as well as bEVs with viable bacteria, were proinflammatory, and killing bacteria with gentamicin reduced cytokine production to baseline levels. Although heat-killed bacteria induced macrophage IL-6 production, heat-killed EVs did not. Additionally, we found that human and bacterial DNA associated with bEVs, but not heat-killed EVs, and that the DNA association could be partially decreased by disrupting electrostatic interactions. We investigated the potential for DNA isolated from EVs (EV-DNA) or EVs to cause inflammation. Although liposomal encapsulation of EV-DNA increased IL-6 production from baseline by 7.5-fold, treatment of bEVs with DNase I had no effect on IL-6 and IL-1 production, suggesting that the DNA did not contribute to the inflammatory response. Filtered EVs, which lacked DNA and associated bacteria, exhibited less proinflammatory activity relative to bEVs, and enhanced macrophage expression of CD86 and HLA-DR. Ultimately, we show that bEVs isolated by differential centrifugation co-purify with bacteria and DNA, and studying their concerted activity and relative contribution to immune response is important to the study of host-pathogen interactions.

In response to several types of bacteria, as well as pharmacological agents, neutrophils produce extracellular vesicles (EVs) and release DNA in the form of neutrophil extracellular traps (NETs). However, it is unknown whether these two neutrophil products cooperate to modulate inflammation. Consistent with vital NETosis, neutrophils challenged with S. aureus, as well as those treated with A23187, released significantly more DNA relative to untreated or fMLF-treated neutrophils, with no lysis occurring for any condition. To test the hypothesis that EVs generated during NETosis caused macrophage inflammation, we isolated and characterized EVs from A23187-treated neutrophils (A23187-EVs). A23187-EVs associated with neutrophil granule proteins, histone H3, transcription factor A, mitochondrial (TFAM), and nuclear and mitochondrial DNA (mtDNA). We showed that DNA from A23187-EVs, when transfected into macrophages, led to production of IL-6 and IFN-α2, and this response was blunted by pre-treatment with the STING inhibitor H151. Next, we confirmed that A23187-EVs were engulfed by macrophages, and showed that they induced cGAS-STING-dependent IL-6 production. In contrast, neither EVs from untreated or fMLF-treated cells exhibited pro-inflammatory activity. Although detergent-mediated lysis of A23187-EVs diminished IL-6 production, removal of surface-associated DNA with DNase I treatment had no effect, and A23187-EVs did not induce IFN-α2 production. Given these unexpected results, we investigated whether macrophage mtDNA activated the cGAS-STING signaling axis. Consistent with mitochondrial outer membrane permeabilization (MOMP), a defined mechanism of mtDNA release, we observed macrophage mitochondrial membrane depolarization, a decrease in cytosolic Bax, and a decrease in mitochondrial cytochrome c, suggesting that macrophage mtDNA may initiate this EV-dependent signaling cascade. All together, these data demonstrate that A23187-EVs behave differently than transfected NET- or EV-DNA, and that neutrophil-derived EVs could be used as a model to study NF-κB-dependent STING activation.

During 2013, multiple tornadoes occurred across Australia, leading to 147 injuries and considerable damage. This prompted speculation as to the frequency of these events in Australia, and whether 2013 constituted a record year. Leveraging media reports, public accounts and the Bureau of Meteorology observational record, sixty-nine tornadoes were identified for the year in comparison to the official count of thirty-seven events. This identified set and the existing historical record were used to establish that, in terms of spatial distribution, 2013 was not abnormal relative to the existing climatology, but numerically exceeded any year in the Bureau record. Evaluation of the environments in which these tornadoes formed illustrated that these conditions included tornado environments found elsewhere globally, but generally had a stronger dependence on shear magnitude than direction, and lower lifted condensation levels. Relative to local environment climatology, 2013 was also not anomalous. These results illustrate a range of tornadoes associated with cool season, tropical cyclone, East Coast low, supercell tornado and low shear/storm merger environments. Using this baseline, the spatial climatology from 1980-2019 as derived from the non-conditional frequency of favorable significant tornado parameter environments for the year is used to highlight that observations are likely an underestimation. Applying the results, discussion is made of the need to expand observing practices, climatology, forecasting guidelines for operational prediction, and improve the warning system. This highlights a need to ensure that the general public is appropriately informed of the tornado hazard in Australia, and provide them with the understanding to respond accordingly.