Andes dissected flank with its own characteristics. In addition, the geological formations that affect the water quality of the Caplina Basin were identified based on the geological cartography. The sources with the greatest influence on water quality were determined. These included the Aruma and Paralocos geothermal springs which produce water with high contents of arsenic, lead, and sodium, in concentrations exceeding the Environmental Quality Standard (ECA, Spanish acronym), and high iron concentration in the water channel from the Barroso Chico spring to the Ancoma Creek. The relationships among the geology, climate, and hydrology were identified, which reflect changes in the concentrations of the elements in the water during seasons with low water levels versus those with high levels.

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The coastal aquifer La Yarada has anthropogenic and geogenic contamination that adversely affect the quality of groundwater for population and agricultural use. In this scenario, multivariate statistical methods were applied in 20 physicochemical and isotopic parameters of 53 groundwater pumping wells in October 2020, with the aim of characterizing the hydrogeochemical processes that dominate the groundwater of the coastal aquifer and the factors that cause them to optimize the effective management of water resources, delimiting areas affected by more than one salinization process. The samples were grouped into three clusters (C1, C2, and C3) with cluster analysis, the spatial distribution of C2 and C3 (reclassified in stiff diagrams), evidenced hydrogeochemical facies associated with the flow and recharge directions governed by the structural lineaments (NE-SO), favoring some areas more than others, arising different facies and hydrogeochemical processes. Factor analysis was applied from three different approaches: (1) main elements, (2) trace elements, and (3) physicochemical and isotopic parameters; exposing 6 distinguishable hydrogeochemical processes in the aquifer and factors that cause them: (i) salinization—marine intrusion, (ii) fertilizer leaching and dissolution of (Ca2+, Mg2+), (iii) wastewater mixture (NO3−), (iv) reducing conditions (Fe, Mn, Al), (v) contributions of (B, Sr), (vi) conservative mixtures and dissolution (As, F). It was validated with water quality indices (WQI) according to the national limits, delimiting 67 km2 parallel to the coast with “bad” to “very bad” quality for human consumption and unsuitable for irrigation according to the Wilcox diagram thus pre-treatment in this area is indispensable.

Global projections of climate change indicate negative impacts on hydrological systems, with significant changes in precipitation and temperature in many parts of the world. As a result, floods and droughts are expected. This article discusses the potential effects of climate change and variability on the maximum precipitation, temperature, and hydrological regime in Devil’s Creek, Tacna, Peru. The outputs of precipitation and daily temperature of fifteen regional climate models were used for the RCP4.5 and RCP8.5 emission scenarios. The methodology used includes the bias correction and downscaling of meteorological variables using the quintiles mapping technique, hydrological modeling, the evaluation of two emission scenarios, and its effect on the maximum flows of the stream. The results of the multi-model ensemble show that the maximum annual precipitation will probably increase by more than 30% for the RCP4.5 and RCP8.5 scenarios for the 2021–2050 period relative to the 1981–2005 period. Likewise, as expected, the maximum flows could increase by 220% and 154% for the RCP4.5 scenarios for the 2021–2050 and 2051–2080 terms, respectively, and 234% and 484% for the RCP8.5 scenarios and for the 2021–2050 and 2051–2080 terms, respectively, concerning the recorded historical value, increasing the probability of flood events and damage in populations located downstream.

The La Yarada aquifer is the primary water resource for municipal, irrigation, and industrial uses in the semi-arid Tacna, Peru. Presently, over-pumping has caused severe groundwater management problems, including the abandonment of saline water wells. This study presents multivariate analysis and chemical–isotopic trends in water to investigate seawater intrusion and hydrogeological processes affecting water quality. The chemical and isotopic analysis of water samples, collected in two campaigns in the dry (August 2020) and wet (November 2020) seasons, together with the 1988 data, were evaluated with a mixing model, cluster, and factor analysis. The hydrochemical and isotopic mixing model suggested the formation of a wedge with 20% seawater intrusion. The heterogeneity of piezometric map isolines corroborates the wedge formation associated with the groundwater movement. The spatial distributions of factors, FA1 and FA2, suggest two processes of seawater front movement: dispersion (diffusion) of chemical elements and different types of water mixing, respectively. At the edge of the La Yarada aquifer, the water head was relatively low, permitting seawater and freshwater mixing. On the other hand, along the sea-land boundary, the water head of the La Yarada aquifer was relatively high, avoiding seawater and freshwater mixing; however, the chemical species were migrating from the seawater to the groundwater due to the diffusion processes. The cluster 4 samples are in the region corresponding to the isotopic mixing process represented by the FA2, while cluster 4 describes the chemical diffusion process represented by the FA2. Thus, the integrated approach is helpful to assess the seawater intrusion mechanisms in coastal aquifers in a semi-arid region.

Climate change, as well as the appearance of pests and diseases, are affecting olive plantations (Olea europaea L.) and the production of olives in the world, therefore, there is an urgent need for tools to help us identify the spatial and temporal evolution of the olive groves, regarding the attack of pests, in this case, the Orthezia olivicola and the olive borer Phloeotribus scarabaeoides. In this work, we use information from freely available satellite images that allowed us to carry out spatial and temporal analysis and the combination of vegetation indices. For the studied area, according to the values of the NDVI (Normalized Difference Vegetation Index), it was identified that the surface of diseased plants ranges from 42% to 68%, moderately healthy plants from 2% to 18%, and the state of the plantation considered as very healthy plants with a tendency to zero, which means that practically 100% of the olive trees are affected by some level. The temporal variation of the NDVI, DVI, SAVI, GNDVI, EVI2, and MSAVI indices, allowed us to establish the states of affectation as mild, moderate pest attack, the severity of the pest attack added to the water deficit, and very strong pest attack and state of permanent wilting.

Chimeric proteins are used to study protein domain functions and to recombine protein domains for novel or optimal functions. We used a library of chimeric integrase proteins to study DNA integration specificity. The library was constructed using a directed shuffling method that we adapted from fusion PCR. This method easily and accurately shuffles multiple DNA gene sequences simultaneously at specific base-pair positions, such as protein domain boundaries. It produced all 27 properly-ordered combinations of the amino-terminal, catalytic core, and carboxyl-terminal domains of the integrase gene from human immunodeficiency virus, prototype foamy virus, and Saccharomyces cerevisiae retrotransposon Ty3. Retrotransposons can display dramatic position-specific integration specificity compared to retroviruses. The yeast retrotransposon Ty3 integrase interacts with RNA polymerase III transcription factors to target integration at the transcription initiation site. In vitro assays of the native and chimeric proteins showed that human immunodeficiency virus integrase was active with heterologous substrates, whereas prototype foamy virus and Ty3 integrases were not. This observation was consistent with a lower substrate specificity for human immunodeficiency virus integrase than for other retrovirus integrases. All eight chimeras containing the Ty3 integrase carboxyl-terminal domain, a candidate targeting domain, failed to target strand transfer in the presence of the targeting protein, suggesting that multiple domains of the Ty3 integrase cooperate in this function.