The plasma concentration of the major vitamin D metabolites; 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, and 24,25-dihydroxyvitamin D were measured during pregnancy and lactation in the adult female rat. The concentrations of these metabolites were also measured in rat pups during lactation and after weaning.. The plasma concentration of 1,25-dihydroxyvitamin D in the adult female increases from a control value of 26 pg/ml to 86 pg/ml during the latter stages of pregnancy, reaches a peak of 158 pg/ml during lactation, and then returns to control levels by 3 weeks postweaning. Plasma concentrations of 24,25-dihydroxyvitamin D fall dramatically during pregnancy from a control level of 3.9 ng/ml to 1.6 ng/mI remain low during lactation, and return to control levels by 3 weeks postweaning. In the neonatal rat pup at 14 days postpartum, 1,25-dihydroxyvitamin D plasma concentration is 25 pg/ml and 24,25-dihydroxyvitamin D concentration is 2.8 ng/ml. By day 25 postpartum, 1,25-dihydroxyvitamin D concentrations reached levels of 101 pg/ml, whereas 24,25-dihydroxyvitamin D concentrations fell to 1.9 ng/ml.The metabolism of vitamin D and its involvement in normal calcium/phosphate metabolism are well documented (1-5). Dietary changes in calcium and phosphate are compensated for by changes in the vitamin D endocrine system in such a way as to maintain a normal calcium and phosphate balance.Pregnancy and lactation bring about dramatic changes in the calcium/phosphate homeostatic mechanism (6). Intestinal calcium transport (7) and bone resorption (8) increase to meet fetal and neonatal calcium and phosphate requirements. The subsequent changes in vitamin D metabolism, however, have not been exhaustively described. Recent reports indicate that the most active hormonal form of vitamin D, 1,25-dihydroxyvitamin D [1,25-(OH)2D], is increased during pregnancy and lactation (9, 10). There is also strong evidence that there are fundamental differences between maternal and fetal metabolism of vitamin D (11). These findings underscore the importance of establishing the plasma concentrations of the major vitamin D metabolites and their relation to each other during pregnancy and lactation. In this paper we report the plasma concentrations of 25-hydroxyvitamin D (25-OH-D), 1,25-(OH)2D, and 24,25-dihydroxyvitamin D [24,2D] during pregnancy, lactation, and the postweaning period in the adult rat as well as the levels of these metabolites in the neonatal rat pup. MATERIALS AND METHODSFemale Holtzman rats (Holtzman Rat Co., Madison, WI) were obtained as weanlings and maintained on a diet containing 0.44% calcium and 0.30% phosphorus (12). In addition, animals were given 25 international units of vitamin D per day in 0.1 ml of cottonseed/soybean oil (Wesson). At 130 days of age, the females were mated with normal male breeders from Holtzman. The presence of sperm in vaginal smears was used to establish the first day of pregnancy.At days 18 and 20 of pregnancy, at day 14 of lactation, at the time of weaning which was set at 25 days ...
To investigate the biological importance of 24R-hydroxylation of 25-hydroxyvitamin D to the early development of rats, the potency of 24,24-difluoro-25-hydroxyvitamin D3 had been compared to that of 25-hydroxyvitamin D3 in young rat pups born to vitamin D-deficient mothers. 24,24-Difluoro-25-hydroxyvitamin D3 and 25-hydroxyvitamin D3 were equally active in stimulating active calcium transport in the intestine, maintaining normal concentrations of calcium and phosphorus in the plasma and promoting bone growth and mineralization. These results provide strong evidence that the presence of a hydroxyl group at the 24 position of vitamin D3 is not required for the maintenance of calcium-phosphate homeostasis during growth and in the development and mineralization of bone.
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