The examination of sperm concentration in the laboratory is the calculation of the number and motility using a microscope or using a device. There are still some clinicians who doubt the accuracy of the sperm count results using a semen analyzer rather than using the manual method. This study aims is to determine the differences of the sperm concentration examination between the manual method and the automatic method. Subjects in this study were patients who carried out semen analysis tests at the Clinical Pathology Laboratory of RSIA "Restu Ibu" Sragen from June to August 2020. The object of this research is the examination of sperm concentration, using a manual method using a hemocytometer and an automatic method using the LensHooke ™ SQA X1 Pro. The results of statistical tests using the Mann Whitney methods show that the significance value (p) was 0.960, which means that there was no difference in the results of the sperm concentration examination between the manual method and the automatic method. Result of this research shows that there is no weakness or significant difference if compared between manual and automatic methods.
Kesehatan merupakan milik setiap manusia tak terkecuali anak-anak. Keadan sehat bagi anak-anak harus mendapatkan perhatian penuh untuk menunjang perkembangan individu. Keadaan sehat pada organ telinga, hidung, dan tenggorokan perlu diwujudkan sebagai salah satu upaya menuju Indonesia Sehat. Siswa SDN Gulon 2 merupakan siswa sekolah di pedesaan yang belum pernah memeriksakan kesehatannya, oleh karena itu diperlukan pemeriksaan telinga, hidung, dan tenggorokan. Pemeriksaan telinga, hidung dan tenggorokan dilakukan dengan bantuan senter dan spatula lidah untuk menilai bagian-bagian pada ketiga organ tersebut. Ditemukan kondisi telinga normal sebanyak 48,5% sedangkan sisanya terdapat serumen; kondisi hidung normal sebanyak 78,7%, sedangkan sisanya rhinitis akut; dan kondisi tenggorokan normal 66,7%, sedangkan sisanya tonsilitis kronis. Tatalaksana dilakukan terhadap telinga yang terdapat serumen apabila memungkinkan. Guru dan siswa sekolah dasar diberikan pendidikan kesehatan tentang kondisi telinga, hidung, dan tenggorokan berkaitan dengan pencegahan maupun tatalaksana selanjutnya apabila keadaan memberat. Kegiatan pengabdian masyarakat perlu dilakukan kepada siswa-siswa di sekolah dasar maupun tingkat lainnya terutama di pedesaan agar mereka memperoleh kesehatan yang optimal.
Introduction: A point mutation of codon 59 (GGC ➞ GAC) of the α2-globin gene, known as haemoglobin (Hb) Adana, contributes to various kinds of α-thalassemia syndrome. This case report described a patient who had heterozygous alpha-thalassemia 3.7 kb deletion and hemoglobin adana. Case report: We report a case of a 17-year-old boy who was referred for the investigation of persistent anemia. His peripheral blood film was consistent with mild hemolytic anemia, without HbH inclusions. Normal HbA2 levels without HbH and HbBart peaks. Both his parents had normal Hb levels, but his mother presented with mild microcytosis. DNA analysis revealed a compound heterozygote for one gene deletion (α-3.7) thalassemia and CD59 (GGC→GAC) mutation in the α2-globin gene. The mother was heterozygous for Hb Adana, and the father was heterozygous for α-3.7. Conclusion: This case report emphasizes the need to consider Hb Adana detection in the absence of HbH inclusions and normal Hb analysis. Therefore, DNA analysis is strongly suggested to confirm the diagnosis and improve the management of thalassemia patients.
Latar belakang : Platelet Rich Plasma (PRP) adalah plasma dalam jumlah sedikit dengan jumlah trombosit banyak, didapatkan melalui sentrifugasi. Trombosit dalam PRP penting untuk penyembuhan luka dan memperbaiki jaringan rusak. Kecepatan sentrifugasi berperan penting dalam memisahkan sel darah merah dengan plasma dan menghasilkan platelet konsentrat. Penelitian ini bertujuan untuk mengetahui perbedaan jumlah trombosit, leukosit dan eritrosit dengan kecepatan sentrifugasi yang berbeda pada pembuatan platelet rich plasma. Metode: Penelitian belah lintang pada orang sehat di laboratorium RSUP Dr. Kariadi Semarang bulan Juli 2018. Darah vena ditampung dalam tabung sitras dan Ethylenediaminetetraacetic acid (EDTA). Darah EDTA untuk pemeriksaan hematologi rutin dengan hematology analyzer, hasil normal dilanjutkan pembuatan PRP dari darah sitras 3,2 % dengan dua metode sentrifugasi, dengan kecepatan 100xg-400x selama 10 menit (metode PRP1) dan kecepatan 540xg-2270xg selama 10 menit (metode PRP2). Kedua produk PRP dilakukan pemeriksaan jumlah trombosit, leukosit dan eritrosit. Data dianalisis dengan uji Kruskall Wallis dan Mann Whitney, signifikan bila p< 0.05. Hasil : Sampel terdiri dari 35 orang responden. Terdapat perbedaan bermakna jumlah trombosit, leukosit dan eritrosit pada pembuatan PRP-1 dan PRP-2 (p<0.001) Simpulan : Terdapat perbedaan jumlah trombosit, leukosit dan eritrosit pada kecepatan sentrifugasi yang berbeda. Kecepatan sentrifugasi pada metode pembuatan PRP disarankan dalam pembuatan PRP. Kata kunci : PRP, trombosit, leukosit, eritrosit Background : Platelet Rich Plasma (PRP) consisting of small amounts plasma with many platelet, which was obtained by centrifugation process. The platelet in PRP are essential for wound healing and repair of damaged tissue. The centrifugation velocity plays an important role in separating red blood cells by plasma and producing concentrated-platelets. This study aims to determine differences the number of platelets, leukocytes and erythrocytes with different centrifugation rates on platelet rich plasma production. Methods: This cross sectional study was conducted on 35 healthy people in the laboratory of Kariadi Hospital Semarang in July 2018. The blood vein were collected in Citrate 3.2 % and Ethylenediaminetetraacetic acid (EDTA) tubes. Sample EDTA for complete blood counts, if the results were normal then continued with PRP preparation in 2 different methods centrifugation (PRP 1 at 100xg-400xg for 10 minutes and PRP 2 at 540xg-2270 xg for 10 minutes). Both of the PRP products were calculated the amounts of platelet, leukocytes, and erythrocytes. The data were analyzed by Kruskall Wallis and Mann Whitney, p < 0.05 was considered as significant. Results : There was a significant difference between PRP-1 and PRP-2 method in platelet counts, leukocytes count and erythrocytes count (p<0.001). Conclusion : There was differences in platelet count, leukocytes count and erythrocytes count with different centrifugation rate. Centrigugation rate on PRP-1 method is recommended in the production of PRP. Keyword PRP, platelets, leukocytes, erythrocytes
Background : HbA1c is one of the parameters that can be useful for diagnosing diabetes mellitus that can occur in obesity. Monocyte as an inflammatory cell is widely studied in relation to diabetes mellitus and obesity. The purpose of this study is to analyze the correlation between HbA1c levels and monocyte percentage in obesity.Methods : A Cross-sectional study of 30 medical students at a private laboratory in Semarang on March – April 2017 was done. The monocyte percentage included incomplete blood count was examined with the Sysmex XS-800i hematology analyzer, while HbA1c level was examined with Hemocue HbA1c 501 System. Spearman test was done for analyzing data with p <0.05 was considered statistically significant. Result and discussion : Median of HbA1c level was 5.8 ± 0.23%. Medians of leukocyte count, lymphocyte percentage, neutrophil percentage, and monocyte percentage were 8,600 ± 339,77 /μl, lymphocyte percentage 33% ± 1.19%, neutrophil percentage 56% ± 1.35%, and monocyte percentage of 9.0% ± 0.26%, respectively. Spearman test showed that correlation value (r) was 0.131 (p = 0.489).Conclusion and suggestion : There was no correlation between HbA1c levels and monocyte percentage in obesity. The increased of monocyte percentage can be used as monitoring and evaluation parameters in obesity that can lead to diabetes mellitus. Further study can be done in an obese population with older age as well as other parameters related to obesity and diabetes mellitus.
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