We identified 126 tumor cell lines established from patients with small cell cancer at the NCI-Navy Medical Oncology Branch from 1977 through 1992. Extensive clinical information was available on 96 patients from whom these cell lines were established. These patients comprised approximately one fourth of the 407 patients treated on prospective therapeutic clinical trials during the same time period. The proportion of tumor cell lines established from previously untreated patients with both limited and extensive stage small cell lung cancer increased during the 16 years of the study (P = 0.008). MYCfamily DNA amplification was present in 16 of 44 (36%) tumor cell lines established from previously treated patients compared to 7 of 52 (1 1%) of tumor cell lines established from untreated patients (P = 0.009). MYC DNA amplification in tumor cell lines established from patients previously treated with chemotherapy continued to be associated with shortened survival (P = 0.001 ). The initiation of a policy to obtain tumor tissue for the purpose of selecting chemotherapeutic agents given to individual patients was associated with an increase in the proportion of patients from whom tumor cell lines could be established for both extensive and limited stage patients (P = 0.0001 and 0.05, respectively). D 1996 wiley-Liss, Inc.Key words: lung neoplasms, oncogenes, drug therapy, mortality, pathologyThree of the members of the MYC family, MYC, NMYC, and LMYC, have been shown to be amplified in tumors and tumor cell lines from patients with small cell lung cancer [l-181. MYC DNA amplification has been the most frequently observed and is associated with a variant form of small cell lung cancer cell lines that have a more rapid growth rate than the classic type [2,19,201. We have previously shown that MYC family DNA amplification is more common in tumors and tumor cell lines derived from small cell lung cancer patients previously treated with combination chemotherapy [7,10,14,21]. established from chemotherapy-treated patients is associated with a shortened survival time [7,141. This supplement has attempted to provide a comprehensive list of all cell lines established at the NCI-Navy and NCI-VA Medical Oncology Branches. We have collected extensive clinical information on nearly all the patients with small cell lung cancer from whom cell lines were established. However, many of our cell lines have appeared in the literature (and included in this supplement) which have not been part of our analysis of MYC family DNA amplification and its association with small cell lung cancer patients' clinical status and course. Tumor cell lines established from patients treated on prospective clinical protocols and patients who have comprehensive data about their treatment have been included in this patient treatment analysis. Patients from whom tumor cell lines were established but not included in this analysis have footnotes explaining why they have been excluded. In addition, we have included references from our previous article...
More than 200 human small cell lung cancer and non-small cell lung cancer cell lines were established over 15 years mainly by utilizing the serum-free, hormone and growth factor supplemented, defined media HITES and ACL4. Use of modified, established cell culture techniques such as the mechanical spillout method for the releasing of cell aggregates from tumor tissue, ficoll gradient centrifugation for the separation of tumor cells from erythrocytes and tissue debris, and an apparatue consisting of a platinum tubing attached to a suction flask for removal of spent medium have greatly contributed to the success in culturing tumor cells. Characterization of these lung cancer cell lines have extended our knowledge of lung cell biology. Studies elucidating the nutritional requirements of lung cancer cell growth may be helpful for the manipulation of these tumors in patients.
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