The purpose of the present study was to evaluate the efficacy of propolis extract in maintaining the viability of human periodontal ligament (PDL) cells, and to radiographically analyze tooth replantation and the adjacent periodontium in dogs after storage in this extract. Human PDL cells were incubated with the experimental media propolis, milk, saliva, Hank's balanced salt solution (HBSS), and Dulbecco's modified Eagles medium (DMEM, positive controls), and distilled water (negative control). Cell viability was determined 0, 1, 3, 6, 12, and 24 h later by colorimetric MTT assay. Thirty incisors from dogs were divided into two storage time blocks (1 and 3 h) and were maintained in the experimental media. HBSS served as a positive control, and dry teeth (on gauze) as a negative control. The replanted teeth were radiographed once per month for 6 months. The radiographic images were standardized by the shortening/lengthening factor, and were both qualitatively and quantitatively analyzed. The in vitro results showed that the efficacy of propolis in maintaining functional viability of PDL cells was similar to that of milk. Propolis and milk were significantly better than controls from the 6-h time period. The in vivo results showed that teeth maintained in propolis medium exhibited replacement resorption with significant reduction in tooth length, similar to teeth maintained in saliva and dried teeth. This resorption was less intense with the 3-h storage time than the 1-h storage time. Conditions close to normal were found in teeth maintained in milk, similar to the HBSS control. Therefore, although propolis was effective in maintaining the viability of human PDL cells, resorption of the tooth replantation in dogs occurred under these experimental conditions.
The Gorlin-Goltz syndrome, also known as nevoid basal cell carcinoma syndrome (NBCCS), is an infrequent multisystemic disease inherited in a dominant autosomal way, which shows a high level of penetrance and variable expressiveness. It is characterized by keratocystic odontogenic tumors (KCOT) in the jaw, multiple basal cell nevi carcinomas and skeletal abnormities. This syndrome may be diagnosed early by a dentist by routine radiographic exams in the first decade of life, since the KCOTs are usually one of the first manifestations of the syndrome. This article paper reports the case of a patient, a 10-year-old boy with NBCCS, emphasizing its clinical and radiographic manifestations. This study highlights the importance of health professionals in the early diagnosis of NBCCS and in a preventive multidisciplinary approach to provide a better prognosis for the patient.
The light-curing technique is relevant to reduce the degree of polymerization shrinkage, improving clinical and esthetic success of composite resin restorations.Objective:To evaluate in vitro the effect of four light-curing techniques on depth of cure of a composite resin.Material and Methods:Ten specimens of a composite resin were made in cylindrical cavities prepared in PVC plates (3.0 X 7.0 mm) for each light-curing technique. Four photoactivation methods were investigated: stepped, ramped, pulse-delay and traditional. Specimens were longitudinally sectioned and polished for microhardness measurements (kg/mm2), which were made at 0.1, 1.0, 2.0 and 4.0 mm from the irradiated surface. Data were subjected to ANOVA and Tukey's test.Results:The effect of factors studied (curing method and distance from the surface) and the interaction of these factors was statistically significant (p<0.05). The traditional method of cure provided higher microhardness values (69.6 ± 2.5) than the stepped (63.5 ± 3.1) and pulsed (63.9 ± 3.2) methods at all depths evaluated, but it did not differ from the ramped method (66.7± 4.4) at 0.1 and 1.0 mm of depth.Conclusion:All techniques employed provided satisfactory cure of the composite resin up to the depth of 2.0 mm from the irradiated surface.
The aim of this study was to evaluate the healing process of intramembranous (IM) and endochondral (EC) bone grafts under low‐level laser therapy (LLLT). Male rabbits underwent onlay autogenous bone grafts (1 cm in diameter) retrieved from the calvaria and iliac crest and fixed on parietal bones, divided into four groups: Calvaria (C), Iliac (I), Calvaria + LLLT (C+L), and Iliac + LLLT (I+L). Animals from C+L and I+L Groups had their grafts exposed to LLLT (AlGaAs–808 nm, CW, 30 mW, 0.028 cm2 average laser beam area), 15 s irradiation time (16 J/cm2 per point–total of 64 J/cm2 per session). After 7, 14, 30, and 60 days, grafts were retrieved and resorption pattern analyzed by means of morphometry and TRAP‐positive osteoclasts detection. Differences in the resorption levels of iliac grafts were observed, presenting 40% in I group against 8% in I+L grafts at the 14th day of evaluation (P < 0.05). After 30 days, resorption was maintained at 41% in I group, whereas I+L presented 15% in the same period (P = 0.0591). No significant differences were noted in the rates of calvaria grafts resorption in all periods. A significant higher number of osteoclasts on the grafts' surface was observed in C+L Group at day 30, in comparison with C group. In I+L Group, prevalence of osteoclasts was marked at day 7 (P < 0.05) in comparison to I Group. In general, it was concluded that biomodulative effects of LLLT did not significantly affect healing and resorption processes of autogenous bone grafts from EC and IM origins. Microsc. Res. Tech. 75:1237–1244, 2012. © 2012 Wiley Periodicals, Inc.
O Granuloma Piogênico é uma lesão reativa comum, que se caracteriza por uma proliferação excessiva de tecido conjuntivo como resposta a uma agressão. Pode aparecer tanto em pele como em mucosas, sendo a boca o local mais afetado (CAPELARI et al., 2009). Na cavidade bucal, caracteriza-se clinicamente por crescimento tecidual exofítico, séssil ou pediculado, de coloração eritematosa à acastanhada, geralmente ulcerada e com sangramento espontâneo. A exérese cirúrgica da lesão, associada à remoção dos fatores irritativos locais, como as doenças periodontais, biofilme e cálculos dentários (REYES et al., 2008), representam o tratamento de escolha. O seguinte trabalho tem o propósito de apresentar um caso clínico de paciente do gênero feminino, 46 anos, feoderma, encaminhada ao Serviço de Cirurgia do curso de Odontologia do Centro Universitário de Maringá, com a presença de uma extensa lesão na região posterior da maxila direita. Clinicamente, apresentava-se como uma massa nodular, de superfície moriforme, base pediculada, coloração avermelhada, revestida parcialmente por membrana amarelada, assintomática, medindo aproximadamente 16,0mm x 8,0mm. Ao exame radiográfico, observou-se presença de raízes residuais sob a lesão, corroborando com a hipótese de Granuloma Piogênico. O tratamento consistiuse na remoção total da lesão, e dos possíveis agentes causais, entre eles, as raízes residuais.Palavras-chave: Estomatologia; Granuloma Piogênico; Patologia bucal.
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