Background: Our understanding of the interaction between the host immune system and cancer has evolved rapidly over the previous 10 years due to the clinical success induced by checkpoint inhibition. Previous immunologic work can now be realized in combination strategies. Modified Vaccinia Ankara (MVA) offers significant opportunities due to its natural induction of innate and adaptive immunity, large payload, and excellent safety profile. Methods: MVA vectors were administered subcutaneously (SC), intravenously (IV) or intratumorally (IT) into tumor-bearing mice. Cytokine secretion profile in serum was assessed by Luminex analysis. NK and T cell infiltration and activation in various organs and cytolytic activity against target cells were determined by flow cytometrybased assays. PD-1 immune checkpoint blockade (ICB), low dose cyclophosphamide, tumor-targeting Abs or 15 Gy radiotherapy were administered along with IV MVA. Results: Recombinant MVA (rMVA) in which tumor antigens and costimulatory molecules are encoded can be customized for maximal effect by route of administration. rMVA administered intravenously (IV) causes superior induction of antigen specific T cells, cytokines and NK cells than previously seen with subcutaneous or intramuscular routes. Encoding CD40L in addition amplifies the effects and efficacy improves in relevant models. This is dependent on T cells and NK cells, indicating a potential solution to one tumor-resistance mechanism, MHC loss and/or mutation. Furthermore, the combination of rMVA with tumor targeting antibodies, checkpoint inhibition, radiotherapy or chemotherapy often showed additional synergistic therapeutic effects. Administration of MVA alone intratumorally (IT) causes innate immune activation through toll like receptors (TLRs) as well as the cGAS / STING pathway. Recombinant antigen encoding rMVA improves systemic and local antigen-specific T cell responses. These effects can be bolstered by encoding certain costimulatory molecules. Conclusions: IV and IT recombinant MVA may offer off the shelf solutions to resolve many of the host -tumor immunity interactions that result in lack of efficacy of checkpoint inhibition alone in most patients. Bavarian Nordic plans to initiate clinical trials with existing agents in 2019 applied IV and IT and will create novel constructs to maximize clinical effect, planned to initiate in 2020 and beyond. Legal entity responsible for the study: Bavarian Nordic, Inc.

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Esta pesquisa teve por objetivo analisar o impacto das atividades remotas nos cursos de Educação Física da Universidade da Região de Joinville/SC - Univille, durante o período pandêmico. O método adotado para investigação foi o descritivo e transversal. Participaram do estudo 110 estudantes, todos dos cursos de graduação em Educação Física da Univille. Como instrumento de pesquisa foi utilizado um questionário, organizado na plataforma Google Forms®, composto de 12 questões afrmativas, no modelo Escala Likert para mensuração das respostas. As perguntas foram organizadas em três categorias: Aprendizado, Caminho Profssional e Reconhecimento Intrínseco. Os dados depois de coletados foram organizados em uma planilha do programa SPSS® 16.0. Após a determinação da normalidade da distribuição dos dados, por meio do teste de Kolmogorov-Smirnov, foi aplicado o teste de correlação de Pearson e os valores de p<0,05 foram considerados signifcativos. Ao efetuarmos a análise estatística em cada categoria, os resultados revelaram cinco associações lineares signifcativas moderadas. Uma no domínio Aprendizado (p-valor<0,002), duas no Caminho Profssional (p-valor<0,0003 e p-valor<0,003), e duas no Reconhecimento Intrínseco (p-valor<0,005 e p-valor<0,002). Para a análise entre categorias foram encontradas sete correlações moderadas. Duas no componente “Aprendizado e Caminho Profssional” (p-valor<0,004 e p-valor<0,002), três no “Aprendizado e Reconhecimento Intrínseco” (p-valor<0,005, p-valor<0,004 e p-valor<0,001), e duas na “Caminho Profssional e Reconhecimento Intrínseco” com o mesmo valor (p-valor<0,003). Diante das evidências encontradas, foi possível constatar por intermédio do predomínio de associações lineares signifcativas, que as atividades remotas difcultaram o processo ensino-aprendizagem para os acadêmicosdos cursos de Educação Física, durante a pandemia.

Objective: Identify the factors that influence the adherence of Resisted Training programs in gyms located in the city of Guaratuba, Paraná/BR. Methodology: The design of the present study is cross-sectional. A total of 100 Resisted Training practitioners of both genders, aged between 18 and 75 years, participated in the research. The location for data collection included four gyms located in the city of Guaratuba-PR. Considering the nature of the research, it was chosen to apply a questionnaire adapted from Morales (2002), composed of opened and closed-ended questions. The instrument was composed with exposure variables of sociodemographic, economic, environmental factors, self-perception of lifestyle and health, stress levels indicators and physical activity. The SPSS® 16.0 program was used for statistical treatment. Considering the non-normality of the data distribution through the Kolmogorov-Smirnov test, Spearman’s correlation test was used and values of p<0.05 were considered significant.  Results: The present study showed three significant moderate correlations, demonstrating a joint variation of tobacco use with alcohol (0.655 with p-value<0.05), intake of high alcoholic beverages over others (0.802 with p-value<0.05), and men’s weekly workload (0.62 with p-value<0.05).  Conclusion: According to the obtained data, it’s possible to conclude through the significant moderate correlations, that the sociodemographic aspects and the identification of some behavioral risk factors related to the use of tobacco and alcoholic beverages, can influence the adherence to the training programs in gyms.

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