The Effect of Pre‐irradiation on the Content of Growth Substances in Xi‐Plants of Brassica oleracea var. acephala. The auxin content of growth stimulated young plants (Brassica oleraceav ar. acephala) of the X2‐generation, originating from X‐ray irradiated seeds (75 kR) was determined. Methanolic extracts were separated on thin‐layer‐chromatograms, which were analyzed by ultra‐violet light and various chemical reactions. Quantitative determinations of growth substances were made using the Avena‐coleoptile‐cylinder‐test. The following results were obtained: 1. X2‐plants had a higher content of IAN compared with unirradiated control samples; 2. IAAm was detectable in only two out of 40 experiments; 3. Two fluorescent substances were detected: a hydroxycinnarmc acid derivative and a long chained unsatu‐rated ketone or aldehyde, which is still unknown.
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