produced by the endometrium, acts as an agonist in the myometrium, inducing contractions by activating FPs receptors and mobilizing intracellular Ca 2+. Meanwhile, the PGE2 activity in the myometrium depends on the type of receptor-activated. The PGE2 binding to EP1 and mobilizing the intracellular Ca 2+ , or to EP3 and modulating cAMP and IP3, produces contractions. On the other hand, the binding of PGE2 to EP2 or EP4 induces relaxation via elevating intracellular cAMP accumulation 10-12. The direct involvement of prostaglandins in the pain pathways associated with menstrual disorders, mainly the PGE2, should notbe discarded 8. Depending on the magnitude of the menstrual disorder, non-steroidal anti-inflammatory drugs are used to block the synthesis of PGs and control the clinical conditions. However, this therapeutic choice produces many side effects, restricting its use for long periods. Considering this, ethnomedicine has been proposed in the present study as an alternative treatment for menstrual problems. Several authors have reported the existence of the modulator effects of flavonoids on the regulation of cytokines, prostaglandins and other mediators that drive immune and inflammatory reactions 13-18. Citrus fruits and juices (oranges, mandarins, grapefruits, lemons, bergamots, and limes) are an important source of flavonoids, mainly glycosylflavonoids 19,20. Among a variety of compounds present in citrus, luteolin, for example, can modulate the expression of COX-2, nitric oxide synthase, and inflammatory cytokines 21. Hesperitin, naringenin and, rutin inhibit the COX activity as well as PGE2 production 22-24. Naringin acts on the immune system to prevent tissue damage, while naringenin can inhibit key enzymes in the oxidation of fatty acids, as well as the NF-κβ transcription factor, reducing the production of pro-inflammatory cytokines 25,26. Diosmin and hesperidin possess inhibitory activity over E2 and F2α prostaglandins 27. The combination of hesperidin, nobiletin and tangeretin show a potent suppression over iNO2, TNF-α, IL-1β, and IL-6 cytokines 28. Furthermore, in the human body, the glycosylflavonoids can be converted into their aglycone form, which has shown strong antioxidant and anti-inflammatory activities when compared to the glycosyl form 29. Based on this knowledge, our research group conducted a pilot study in 2014 to analyze the citrus-therapy effects of Tahiti lime (Citrus latifolia) juice (TLJ) in patients with menstrual disorders. The results showed that this Citrus reduced the duration and intensity of excessive bleeding, the occurrence of dysmenorrhea and the presence of clots 30. The choice of Tahiti lime was based on preliminary empirical tests that showed the best results for Citrus latifolia over other limes species. In this pilot, a gynecologist used different types of lime juice during diverse menstrual cycles and identified an advantage in the activity of C. latifolia. This pilot also showed that the juice from one lemon fruit was capable to reduce the menstrual bleeding soon...