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The increase in bacterial resistance and decreased effectiveness of antibacterial agents has forced researchers to look for new antibacterial agents from environmentally friendly sources such as essential oils and oil extracts. The functional group of the metabolites present in the essential oils or plant extract and the synergy effects between them play an important role in the biological activity and can be the principal factor affecting the antibacterial effect. All of these bioactive oils showed the same action mechanism, and the best way to implement them is by extracting them without changing their original properties, whereby the characterization and evaluation of the compounds are important steps. All of these themes are extensively reviewed, analyzed, and discussed in this work.