The trigeminal nerve (V) is the fifth and largest of all cranial nerves, and it is responsible for detecting sensory stimuli that arise from the craniofacial area. The nerve is divided into three branches: ophthalmic (V1), maxillary (V2), and mandibular (V3); their cell bodies are located in the trigeminal ganglia and they make connections with second-order neurons in the trigeminal brainstem sensory nuclear complex. Ascending projections via the trigeminothalamic tract transmit information to the thalamus and other brain regions responsible for interpreting sensory information. One of the most common forms of craniofacial pain is trigeminal neuralgia. Trigeminal neuralgia is characterized by sudden, brief, and excruciating facial pain attacks in one or more of the V branches, leading to a severe reduction in the quality of life of affected patients. Trigeminal neuralgia etiology can be classified into idiopathic, classic, and secondary. Classic trigeminal neuralgia is associated with neurovascular compression in the trigeminal root entry zone, which can lead to demyelination and a dysregulation of voltage-gated sodium channel expression in the membrane. These alterations may be responsible for pain attacks in trigeminal neuralgia patients. The antiepileptic drugs carbamazepine and oxcarbazepine are the first-line pharmacological treatment for trigeminal neuralgia. Their mechanism of action is a modulation of voltage-gated sodium channels, leading to a decrease in neuronal activity. Although carbamazepine and oxcarbazepine are the first-line treatment, other drugs may be useful for pain control in trigeminal neuralgia. Among them, the anticonvulsants gabapentin, pregabalin, lamotrigine and phenytoin, baclofen, and botulinum toxin type A can be coadministered with carbamazepine or oxcarbazepine for a synergistic approach. New pharmacological alternatives are being explored such as the active metabolite of oxcarbazepine, eslicarbazepine, and the new Nav1.7 blocker vixotrigine. The pharmacological profiles of these drugs are addressed in this review.
Highlights d Dopamine projections from the VTA innervate the mPFC d Dopamine release in the mPFC reverses mechanical hypersensitivity in SNI mice d Dopamine release induces conditioned place preference in SNI mice d Dopamine inputs increase activity of mPFC neurons projecting to the vlPAG
Cav3.2 calcium channels are important
mediators of nociceptive
signaling in the primary afferent pain pathway, and their expression
is increased in various rodent models of chronic pain. Previous work
from our laboratory has shown that this is in part mediated by an
aberrant expression of deubiquitinase USP5, which associates with
these channels and increases their stability. Here, we report on a
novel bioactive rhodanine compound (II-1), which was identified in
compound library screens. II-1 inhibits biochemical interactions between
USP5 and the Cav3.2 domain III-IV linker in a dose-dependent manner,
without affecting the enzymatic activity of USP5. Molecular docking
analysis reveals two potential binding pockets at the USP5-Cav3.2
interface that are distinct from the binding site of the deubiquitinase
inhibitor WP1130 (a.k.a. degrasyn). With an understanding of the ability
of some rhodanines to produce false positives in high-throughput screening,
we have conducted several orthogonal assays to confirm the validity
of this hit, including in vivo experiments. Intrathecal delivery of
II-1 inhibited both phases of formalin-induced nocifensive behaviors
in mice, as well as abolished thermal hyperalgesia induced by the
delivery of complete Freund’s adjuvant (CFA) to the hind paw.
The latter effects were abolished in Cav3.2 null mice, thus confirming
that Cav3.2 is required for the action of II-1. II-1 also mediated
a robust inhibition of mechanical allodynia induced by injury to the
sciatic nerve. Altogether, our data uncover a novel class of analgesicswell
suited to rapid structure–activity relationship studiesthat
target the Cav3.2/USP5 interface.
Chlorpyrifos is a pesticide, member of the organophosphate class, widely used in several countries to manage insect pests on many agricultural crops. Currently, chlorpyrifos health risks are being reevaluated due to possible adverse effects, especially on the central nervous system. The aim of this study was to investigate the possible action of this pesticide on the behaviors related to anxiety and depression of offspring rats exposed during pregnancy. Wistar rats were treated orally with chlorpyrifos (0.01, 0.1, 1 and 10mg/kg/day) on gestational days 14-20. Male offspring behavior was evaluated on post-natal days 21 and 70 by the elevated plus-maze test, open field test and forced swimming test. The results demonstrated that exposure to 0.1, 1 or 10mg/kg/day of chlorpyrifos could induce anxiogenic-like, but not depressive-like behavior at post-natal day 21, without causing fetal toxicity. This effect was reversed on post-natal day 70.
A novel missense mutation in the CACNA1A gene that encodes the pore forming α1 subunit of the CaV2.1 voltage-gated calcium channel was identified in a patient with trigeminal neuralgia. This mutation leads to a substitution of proline 2455 by histidine (P2455H) in the distal C-terminus region of the channel. Due to the well characterized role of this channel in neurotransmitter release, our aim was to characterize the biophysical properties of the P2455H variant in heterologously expressed CaV2.1 channels. Whole-cell patch clamp recordings of wild type and mutant CaV2.1 channels expressed in tsA-201 cells reveal that the mutation mediates a depolarizing shift in the voltage-dependence of activation and inactivation. Moreover, the P2455H mutant strongly reduced calcium-dependent inactivation of the channel that is consistent with an overall gain of function. Hence, the P2455H CaV2.1 missense mutation alters the gating properties of the channel, suggesting that associated changes in CaV2.1-dependent synaptic communication in the trigeminal system may contribute to the development of trigeminal neuralgia.
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