Objective: The aim of this study is to evaluate the expression of cycloocygenase-2 (COX-2) in orbital fibroadipose connective tissue in Graves' ophthalmopathy (GO) patients, and investigate the associations between COX-2 expression and GO characteristics. Methods: The orbital fibroadipose connective tissues of 23 cases demonstrating moderate or severe GO, and eight control subjects without any history of thyroid or autoimmune disease were analyzed for COX-2 mRNA expression. Real-time relative quantitative PCR was performed to assess transcripts of COX-2 using the LightCycler. The disease activity was evaluated by the clinical activity score (CAS). The clinical features of GO were evaluated by total eye score (TES) and the cases were divided into two groups; type 1 cases included higher degrees of proptosis with orbital fat volume increase, and type 2 cases included cases with compressive neuropathy and limited extraocular muscle functions. Results: The meanGS.D. disease duration was 5.7G7.1 years. The meanGS.D. CAS and TES of cases were 1.60G1.04 and 7.5G1.8 respectively. The meanGS.D. expression of COX-2 was 0.023G0.013 and 0.010G0.002 in GO cases and controls (PZ0.008), and 0.015G0.073 and 0.029G0.135 in type 1 and type 2 cases respectively (PZ0.007). COX-2 expression showed a statistically significant positive correlation with TES (rZ0.634, PZ0.001), and a negative correlation with the disease duration (rZK0.621, PZ0.002). Conclusions: COX-2 is expressed at higher levels in orbital fibroadipose tissues of GO cases. This showed a positive correlation with increasing severity of orbital disease suggesting possible relation with COX-2 expression and orbital inflammation in GO.European Journal of Endocrinology 155 681-685
A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : To evaluate the expression of cyclooxygenase-2 (COX-2) and Ki-67 in primary and recurrent pterygia. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Pterygium excision together with limbal/conjunctival autografting was performed in primary and recurrent pterygium cases who did not have clinically evident inflammatory findings. The excised tissues were stored at -80 °C for the analysis. Ki-67 expression was determined with only immunohistochemically while COX-2 expression was determined with both immunohistochemistry and real-time polymerase chain reaction, which is a more sensitive method. Normal human conjunctival samples from age-matched donors were used as controls. R Re es su ul lt ts s: : Twelve primary and nine recurrent pterygium tissues were included in the study group. The expression of COX-2/β actin mRNA was not significantly different in primary pterygia, recurrent pterygia and normal conjunctival tissues (0.0082±0.0038, 0.0094 ± 0.0023, and 0.0075 ± 0.0035 respectively (p=0.32). The expression of Ki-67 nuclear antigen was significantly higher both in primary and recurrent cases when compared to control group (5.2±5.0%, 7.1±2.% and 1.29±0.9% respectively (p=0.018 and p= 0.001, respectively). There was no significant correlation between immunohistochemical expression of Ki-67 and COX-2 in primary and recurrent pterygium tissues (p=0.281, r=0.339; p=0.649, r=-0.177, respectively. C Co on nc cl lu us si io on n: : COX-2 expression does not seem to increase in clinically uninflamed primary and recurrent pterygia tissues. Increased Ki-67 expression in primary and recurrent pterygia supports the proliferative nature of the disease which might have implications in regard to treatment options. K Ke ey y W Wo or rd ds s: : Pterygium; Cyclooxygenase 2; Ki-67 antigen Ö ÖZ ZE ET T A Am ma aç ç: : Primer ve nüks pterjiyumda siklooksijenaz-2 (COX-2) ve Ki-67 ekspresyonunun değerlendirilmesi. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : İnflamatuvar bulguları olmayan, primer ve nüks pterjiyum olgularında, limbal konjonktival otogreft yöntemi ile pterjiyum cerrahisi uygulandı. Tüm dokular analiz için -80 °C'de saklandı. Bu dokularda Ki-67 ekspresyonu sadece immünohistokimyasal olarak incelenirken; COX-2 ekspresyonu hem immünohistokimyasal olarak, hem de daha duyarlı bir analiz yöntemi olan gerçek zamanlı polimeraz zincir reaksiyonu yöntemi kullanılarak değerlendirildi. Pterjiyum dokularındaki Ki-67 ve COX-2 ekspresyon düzeyleri, sağlıklı konjonktival dokulardaki ekspresyon düzeyleri ile karşılaştırıldı. Tüm gruplarda Ki-67 ve COX-2 düzeyleri arasındaki korelasyonlar değerlendirildi. B Bu ul lg gu ul la ar r: : On iki primer ve dokuz rekürren pterjiyum olgusu çalışmaya dahil edildi. Ortalama COX-2/β aktin mRNA ekspresyonu primer, nüks pterjiyum ve kontrol konjonktiva dokularında sırasıyla 0.0082±0.0038, 0.0094±0.0023 ve 0.0075 ± 0.0035 idi (p=0.32). Ortalama Ki-67 nükleer antijen immünopozitifliği primer ve nüks pterjiyum dokularında kontrol ...
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